Nanoscale Temperature Mapping and Thermal Regulation of Intracellular Dynamics

项目摘要 温度对所有生命活动都至关重要，并调节生物过程， 不同的层次。细胞不仅通过其独特的 温度敏感性分子机器，而且还适应适当反应 保持其固有功能。尽管温度的基本影响 在生物过程中，细胞产生和利用热量的分子机制 基本上是未知的。我们的目标是开发原子级精确的纳米架构， 刺激器和温度计来研究各种温度敏感分子， 亚细胞水平。该研究将调查时空温度变化， 包括细胞器特异性的产热作用，以发展 温度和细胞功能。拟议的研究将联合收割机纳米架构与 我们最近展示了超快光学光谱，结构表征， 原子到显微镜的多尺度建模框架，用于设计、测量和 分析分子水平的热生物相互作用。新办法将 在微观水平上量化细胞内温度变化， 从宏观层面上的水环境假设。我们预计 新的纳米结构的就业将揭示新的机制和基本 对细胞内温度辅助功能的理解。

Project Summary Temperature is critical to all life activities and regulates biological processes at various levels. Cells not only detect temperature changes through their unique temperature-sensitive molecular machineries but also adapt with appropriate responses to maintain their inherent functions. Despite the fundamental involvement of temperature in biological processes, the molecular mechanism by which cells produce and use heat is largely unknown. Our aim to develop atomically precise nanoarchitectures as both stimulators and thermometers to study various temperature-sensitive molecules down to the sub-cellular level. The study will investigate spatio-temporal temperature variations, including organelle-specific thermogenesis to develop the intrinsic connections between temperature and cell functions. The proposed study will combine nanoarchitectures with our recent demonstrated ultrafast optical spectroscopy, structural characterizations, and atomistic-to-microscope multiscale modeling framework to design, measure, and analyze the molecular-level thermal-biological interactions. The new approach will quantify intracellular temperature changes at the microscopic level in large difference from those assumed at a water environment at the macroscopic level. We expect the employment of new nano-architecture will uncover novel mechanisms and fundamental understandings of intracellular temperature-assisted functions.