Flow Cytometry

摘要--流式细胞仪共享资源 流式细胞仪共享资源为癌症中心成员提供多参数评估和高可靠性 快速丰富不同表型的细胞群体。核心提供全方位服务的高速信元 分拣由专业操作员执行，使用生物安全外壳中的两台BD Aria仪器。用于分析 细胞学，调查人员可以选择由核心员工进行全方位分析，也可以接受培训，然后 独立使用六个分析流式细胞仪中的一个。这些分析细胞仪，全天候可供培训 用户包括两个BD Fortessas，较新的是5激光、18色仪器和ImageStreamX MKII成像流式细胞仪，4个激光，12个成像通道。许多Core的细胞仪都有 自动加载器，能够进行更大规模的实验。分析软件可在核心或通过机构获得 站点许可证。这一广泛使用的核心由CC管理，并得到研究所的支持 仪器设备的采购和维护。在上一个资助期，三大技术平台是 收购，成像流式细胞术作为一项新服务推出。核心总监奥特曼先生和员工 在NCI指定的癌症中心流式细胞仪中心拥有30多年的综合经验，以及 它们在项目的任何阶段提供全面的技术指导，从实验设计到 手稿准备工作。流式细胞术核心被广泛使用，因为在过去5年中，有46 代表所有三个项目的癌症中心实验室，以及Core至少为75个与癌症相关的项目做出了贡献 出版物。

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional site license. This broadly used Core is managed by the CC and receives support from the Institute for instrumentation purchase and maintenance. In the last funding period, three major technology platforms were acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and they provide comprehensive technical guidance at any stage of a project, from experimental design through manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related publications.