K.C. Donnelly Externship - Promotion of Translational/Transdisciplinary Efforts in Graduate and Post-Doctoral Research

项目说明-(摘要) 由于环境中的广泛污染，全氟和多氟烷基物质(PFAS)是一个严重的问题。AS 与其他污染物相比，全氟辛烷磺酸具有持久性、生物累积性和耐降解性。因此，有效的 迫切需要清理全氟辛烷磺酸。利用植物通过植物修复从土壤中提取全氟辛烷磺酸可能是一种实用的方法 接近。我们以前的研究已经调查了大麻植物从土壤中吸收全氟辛烷磺酸，但这一策略主要是 仅限于去除短链氟化分子。我们现有的R01项目调查植物修复 利用新型材料，如超介孔二氧化硅纳米颗粒(UMNS)和碳点(CDS)进行增强。调谐 这些纳米材料的表面化学将增强植物对全氟辛烷磺酸的吸收，发光碳点将 实现对工厂中这些材料的实时跟踪。尽管如此，处理受全氟辛烷磺酸污染的生物质仍然是一项挑战。 微生物降解全氟辛烷磺酸是一项很有前途的技术。这是一个可持续、绿色的过程，需要较低的资本 和维护成本。我们的初步研究实现了全氟辛酸(PFOA)从PFAS中&gt；70%的降解 使用A6微生物还原脱氟后的受污染的大麻生物量。在此，我们建议进一步 用还原脱氟法研究了受污染的大麻植物中全氟辛烷磺酸的微生物降解 酸奶微生物科新种。菌株A6，并评价纳米材料对PFAS降解的影响。 1

Project Description-(Abstract) Per-and polyfluoroalkyl substances (PFAS) are a serious concern due to extensive contamination in environment. As compared with other contaminants, PFAS are persistent, bioaccumulative and resistant to degradation. Therefore, effective clean-up of PFAS is urgently needed. Using plants to extract PFAS from soils through phytoremediation may be a practical approach. Our previous studies have investigated uptake of PFAS by hemp plants from soil, but this strategy is mostly limited to the removal of short-chain fluorinated molecules. Our existing R01 project investigates phytoremediation enhancement with novel materials such as ultra-mesoporous silica nanoparticles (UMNs) and carbon dots (CDs). Tuning the surface chemistry of these nanomaterials will enhance uptake of PFAS by plants and luminescent carbon dots will enable real time tracking of these materials in plants. Still, the disposal of PFAS contaminated biomass remains a challenge. Biodegradation of PFAS by microbes is a promising technology. It is a sustainable, green process that requires low capital and maintenance costs. Our preliminary studies achieved >70% degradation of perfluorooctanoic acid (PFOA) from PFAS contaminated hemp biomass following a reductive defluorination method using A6 microbes. Here, we propose to further investigate the microbial degradation of PFAS in contaminated hemp plants via a reductive defluorination method using a novel Acidimicrobiaceae sp. strain A6 and to assess the effect of nanomaterials on the PFAS degradation. 1