Structure-Function Studies of the Human Cytochrome P450 27 Family

摘要 公社申请行政补充经费，以购买等温滴定量热计。 (ITC)，特别是马尔文MicroCal PEAQ ITC。该仪器将作为一种无标签的方法用于 测定酶-配体相互作用的结合常数(Kd或Ka)和热力学(ΔH和ΔS) 家长奖(1R35GM147276)中描述的实验问题。这一仪器将使我们能够 用我们现有的设备进行更多类型的酶-配体实验，并执行 之前提出的实验更有信心。所有的实验仍符合原来的范围 家长奖的获奖者。

Summary The PI is requesting an administrative supplement to fund the purchase of an isothermal titration calorimeter (ITC), specifically a Malvern MicroCal PEAQ ITC. The instrument will be used as a label-free approach to measure binding constants (Kd or Ka) and thermodynamics (ΔH and ΔS) of enzyme-ligand interactions key to experimental questions described in the parent award (1R35GM147276). This instrument will enable us to perform more types of enzyme-ligand experiments than possible with our current equipment and to perform the previously proposed experiments with greater confidence. All experiments are still in line with the original scope of the parent award.