Enhancing Performance of Antimicrobial Susceptibility Testing (AST) in Support of Vet-LIRN Activities

项目摘要/摘要： 这项比较研究旨在比较Sensititre™Vet AST COMPGP1F/COMPGN1F BioMérieux Vitek®AST-GP81/AST-GN96卡的抗菌板 药敏试验(AST)结果。此方法比较将在公共 细菌伴生的动物病原体，如大肠杆菌、肠球菌、 假中间葡萄球菌、铜绿假单胞菌和其他肠杆菌 分离株。这些细菌病原体可能在同伴动物和人类之间共享； 因此，这些分离株的抗菌素耐药性(AMR)是一个关键问题。AST结果打开 同伴动物对预测治疗结果有重要意义，而且很有价值。 与人类的AMR趋势进行比较。虽然在兽医中被广泛使用 微生物学方面，目前占主导地位的供应商Sensititre™生产的AST平板是 缺乏必要范围的抗菌药来充分检测共同耐药性 这种机制将允许适当地与人类发生的AMR趋势进行比较。 BioMérieux Vitek通常用于人类微生物学实验室；AST卡片 为该系统设计的抗菌素浓度范围包括临床和 对抗菌素耐药性关键指标的流行病学兴趣。尽管 制造商有专为兽医设计的产品，美国很少有实验室使用 用Bio Mérieux VITEK血细胞分析仪检测AST。到目前为止，还没有发表的研究比较 Senssititre™Ast到BioMérieux Vitek这一方法比较的结果将被公布， 它将提供客观数据，供兽医诊断实验室用来确定 哪种方法最适合他们的实验室。这项比较研究将加强 NDSU VDL的AMR监视能力，并将直接支持Vet-LIRN倡议 保护公众健康。这项研究可能会确定可靠且负担得起的第二家供应商 在兽医微生物学方面，这也确保了所有供应商继续提供有用的 兽医产品，并鼓励对提供给兽医的产品进行常规改进 诊断实验室。

Project Summary/Abstract: This comparison study is intended to compare Sensititre™ Vet AST COMPGP1F/COMPGN1F plates to bioMérieux Vitek® AST-GP81/AST-GN96 cards for performance of antimicrobial susceptibility testing (AST) results. This method comparison will be performed on common bacterial companion animal pathogens such as Escherichia coli, Enterococcus species, Staphylococcus pseundintermedius, Pseudomonas aeruginosa, and other Enterobacterales isolates. These bacterial pathogens may be shared between companion animals and humans; therefore, antimicrobial resistance (AMR) of these isolates is a critical concern. AST results on companion animals are significant for prediction of treatment outcome and are valuable comparisons to AMR trends occurring in humans. Although widely used in veterinary microbiology, AST plates manufactured by the currently dominating vendor, Sensititre™, are lacking the necessary range of antimicrobials to adequately detect common resistance mechanisms which would allow for appropriate comparison to AMR trends occurring in humans. The bioMérieux Vitek is commonly used in human microbiology laboratories; AST cards designed for this system incorporate ranges of antimicrobial concentrations of clinical and epidemiological interest for key indicators of antimicrobial resistance. Although the manufacturer has products designed for veterinary use, few laboratories in the United States use the bioMérieux Vitek instrument for AST. To date, there are no published studies comparing Sensititre™ AST to bioMérieux Vitek. Findings of this methods comparison will be published, which will provide objective data that veterinary diagnostic laboratories can use to determine which method is most appropriate for their laboratories. This comparison study will enhance AMR surveillance capabilities of the NDSU VDL and will directly support Vet-LIRN initiatives of protecting public health. This study may identify a reliable and affordable second vendor for AST in veterinary microbiology, which also ensures that all vendors continue offering useful veterinary products and encourages routine improvements of products offered to veterinary diagnostic laboratories.