ADRDL Diagnostic testing support for the FDA CVM VET-LIRN program

4/12/23 项目摘要 南达科他州动物疾病研究和诊断�c实验室(SD ADRDL)将作为一个整体 兽医实验室基因组测序核心实验室�ga�开启和响应 网络(VET-LIRN)。该项目的主要目的是对从工作中获得的分离株进行WGS 与5个合作伙伴来源实验室：密歇根州北卡罗来纳州立大学兽医中心 州立大学兽医诊断�c实验室，明尼苏达州兽医诊断�c实验室，爱荷华州 州立大学兽医诊断�c实验室和俄克拉荷马州动物疾病诊断�c实验室。 这些合作伙伴将培养肠道沙门氏菌、大肠杆菌、假中间葡萄球菌和 来自兽医基质的其他相关生物。一旦发送到SD ADRDL，IDN�TY将被验证fi 通过MALDI-TOF提取基因组�并进行WGS。序列将被上传到NCBI 由VET-LIRN计划指定的生物项目Office。 VET-LIRN的任务是开发AST和WGS检测，将允许 了解兽医样本中的�微生物耐药性。这些知识将被用来 开发基于证据的干预�基因，这些基因已经证明了�基因在耐药性中的减少。 5U18FD006558第1页，共1页

4/12/23 Project Summary The South Dakota Animal Disease Research and Diagnos�c Laboratory (SD ADRDL) will serve as a Whole Genome Sequencing (WGS) core laboratory for the Veterinary Laboratory Inves�ga�on and Response Network (Vet-LIRN). The main aim of this project is to perform WGS on isolates obtained from working with 5 partner source labs: North Carolina State University Center for Veterinary Medicine, Michigan State University Veterinary Diagnos�c Laboratory, Minnesota Veterinary Diagnos�c Laboratory, Iowa State University Veterinary Diagnos�c Laboratory, and Oklahoma Animal Disease Diagnos�c Laboratory. These partners will culture Salmonella enterica, Escherichia coli, Staphylococcus pseudintermedius, and other relevant organisms from veterinary matrices. Once sent to the SD ADRDL, iden�ty will be verified via MALDI-TOF before extrac�ng genomic DNA and performing WGS. Sequence will be uploaded to NCBI bioprojects designated by Vet-LIRN Program Office. Vet-LIRN has been tasked with developing AST and WGS assays that will allow for increased understanding of an�microbial resistance within veterinary samples. This knowledge will be used to develop evidence-based interven�ons that have demonstrated reduc�ons in resistance. 5U18FD006558 Page 1 of 1