GENERATION OF CATALYTIC ANTIBODIES TO NEUTRALIZE COCAINE

吸毒和滥用给社会、医疗保健造成了沉重的代价 制度和经济。一种允许快速、有效的治疗方法 体内成瘾药物的中和具有广泛的应用前景。 20世纪70年代的研究表明，S的常规抗体可以阻断 海洛因对成瘾猴子的影响，但这些抗体的作用缓慢 动力学，只有在低剂量的成瘾剂时才有效 实质性的。然而，一些催化抗体表现出动力学特性。 与自然产生的酶相似，因此可以证明 在治疗毒瘾方面很有效。在拟议的研究研究中， 斯克里普斯研究所的研究合作者将产生 通过酯水解选择性地降解可卡因的抗体。然后 分泌这些抗体的杂交瘤将被鉴定和分离 使用One Cell Systems专有的凝胶微滴(GMD)分泌分析 结合荧光活化细胞分选(FACS)。GMD 这项技术非常适合分离稀有的杂交瘤 预计会产生相对少量的所需催化剂 抗体。针对滥用物质的药物疗法的开发 急需之物。 建议的商业应用： 全身性体内可卡因催化抗体的分离 给药会有很大的治疗作用。新的化验方法 而恢复方法有望在发现一种新的 用于治疗和工业用途的各种催化抗体。

Drug addiction and abuse exacts a heavy toll on society, the health care system, and the economy. A therapy that allows the rapid, effective neutralization of addictive drugs in vivo would enjoy extensive use. Studies in the 1970's showed that conventional antibodies could block heroin's effects in addicted monkeys, but these antibodies had slow kinetics and were effective only with low doses of the addictive substance. However, some catalytic antibodies have exhibited kinetics similar to naturally occurring enzymes and, therefore, could prove effective in treating drug addiction. In the proposed research studies, research collaborators at The Scripps Research Institute will generate antibodies that selectively degrade cocaine via ester hydrolysis. Then hybridomas secreting these antibodies will be identified and isolated using One Cell Systems' proprietary gel microdrop (GMD) secretion assay combined with fluorescent activated cell sorting (FACS). The GMD technology is ideally suited to isolating rare hybridomas that are expected to produce relatively small amounts of the desired catalytic antibodies. Development of pharmacotherapies for substances of abuse are urgently needed. PROPOSED COMMERCIAL APPLICATION: Isolation of cocaine specific catalytic antibodies for systemic in vivo administration would have significant therapeutic utility. The novel assay and recovery method is expected to have wide utility for discovery of a variety of catalytic antibodies for therapeutic as well as industrial use.