ARC DETECTION FOR AMPLIFICATION BASED GENETIC TYPING

这项提议的总体目标是提供一个快速和 一种可靠的基于扩增的基因分型方法 点突变和等位基因单碱基改变。建议的方法 称为ARCTM检测，是专为分析高度 聚合酶可扩增的多态DNA靶点 链式反应(PCR)。该方法可以同时打印数百个 使用来自单个基因的扩增产物 聚合酶链式反应。ARCTM过程依赖于聚合酶的延伸 已固定在固体上的等位基因特异性引物对 支持。ARCTM检测将提供更快和更可靠的 分型结果比等位基因特异性杂交少 技术人员劳动，在人类白细胞抗原配型中应该有广泛的应用， 遗传病诊断、传染病诊断、 取证、基因图谱和突变分析。在第一阶段， 提出的方法的可行性将通过构建 一种用于ALL分型的十二种分析多路ARC探测器样机 利用单次扩增反应检测HL A-DQA1等位基因。 拟议的商业应用ARC TM检测将具有广泛的 在人类白细胞抗原配型、血液和血浆筛查、遗传学研究中的应用 疾病诊断、传染病诊断、癌症 诊断学和基于DNA的取证。我们的首批产品将是 用于组织分型和法医检测的人类白细胞抗原分型系统 工业。

The overall objective of this proposal is to provide a fast and reliable automated method for amplification-based genetic typing of point mutations and allelic single-base changes. The proposed method called ARCTM detection, is designed for analysis of highly polymorphic DNA targets that can be amplified by the polymerase chain reaction (PCR). The method can simultaneously type hundreds of nucleotide positions using the amplified products from a single PCR reaction. The ARCTM process relies on polymerase extension of allele-specific primer pairs that have been immobilized on a solid support. ARCTM detection will provide faster and more reliable typing results than allele-specific hybridization, with less technician labor, and should have broad applications in HLA typing, diagnosis of genetic disease, infectious disease diagnostics, forensics, genetic mapping and mutation analysis. In phase I, the feasibility of the proposed method will be tested by constructing a prototype, twelve-assay, multiplex ARC detector for typing all HLA-DQA1 alleles using a single amplification reaction. PROPOSED COMMERCIAL APPLICATIONS ARC TM detection will have broad applications in HLA typing, blood and plasma screening, genetic disease diagnosis, infectious disease diagnostics, cancer diagnostics, and DNA-based forensics. Our initial products will be HLA typing systems for the tissue-typing and forensic testing industries.