MOLECULAR CONTROL OF ICAM-1 EXPRESSION IN ORAL CANCER

描述：ICAM-1是一种细胞表面蛋白，参与 细胞之间的相互作用，包括在免疫反应和癌症中。它的 高危人乳头瘤病毒和其他病毒上调表达 首席调查员所在机构的调查人员已经建立了三个 口腔癌细胞株具有不同程度的转化，且不同 ICAM-1表达水平。调查员现在将检查 ICAM-1在这些细胞中表达的调控机制。ICAM-1 在每个细胞系中的mRNA积累和半衰期将由以下因素决定 北方斑点。反式激活因子的活性将通过 电泳迁移率改变分析。希望通过对 ICAM-1在转化细胞中表达的调节可能导致 控制肿瘤生长的最佳策略。

DESCRIPTION: ICAM-1 is a cell-surface protein which is involved in cell-cell interactions, including in the immune response and in cancer. Its expression is upregulated by high-risk human papillomaviruses, and other investigators at the principal investigator's institution have set up three oral cell lines with different levels of transformation, and different levels of ICAM-1 expression. The investigator will now examine the mechanisms by which ICAM-1 expression is regulated in these cells. ICAM-1 mRNA accumulation and half life in each cell line will be determined by northern blots. Activities of transactivating factors will be looked for by electrophoretic mobility shift assays. It is hoped that an understanding of the modulation of ICAM-1 expression in transformed cells could lead to optimal strategies in controlling tumor growth.