BARORECEPTOR/HORMONAL INTERACTIONS

这项建议的目的是探讨压力感受器的性质 对清醒动物内分泌功能的影响。 重点是 神经垂体轴，因为有证据表明，有一个互惠的 压力感受器神经与中枢肽能系统的关系。 实验旨在验证压力感受器输入具有 对血管加压素和催产素分泌的抑制作用， 通过脑干去甲肾上腺素能输入。 窦主动脉去神经术将用作 模型，探讨传入输入的去除对 肽能系统。 具体目的是：1）确定 压力感受器去神经对大鼠心血管和内分泌功能的影响 清醒大鼠 血浆血管加压素和 将评价催产素和心血管参数; 2）评价 压力感受器失神经支配的激素和心血管反应性 使用刺激或抑制分泌的范例的动物; 3） 检查去神经动物中枢反应性的变化 使用体内微透析系统将刺激物递送至 室旁区和视上区; 4）确定机制， 压力感受器输入通过评估影响来改变肽分泌 脑干或下丘脑病变和选择性神经去神经 内分泌和心血管反应;和5）检查 下丘脑/脑干的相互作用，通过确定 室旁核小细胞区的特异性病变 原子核 这项调查将提供关于这些因素之间相互作用的信息。 自主神经系统和内分泌下丘脑， 在心血管液体/电解质平衡方面。

The objective of this proposal is to explore the nature of baroreceptor influences on endocrine function in the conscious animal. The focus is on the neurohypophyseal axis since evidence shows that there is a reciprocal relationship between baroreceptor nerves and central peptidergic systems. Experiments are designed to test the hypothesis that baroreceptor input has inhibitory effects on vasopressin and oxytocin secretion which are mediated by brain stem noradrenergic input. Sinoaortic denervation will be used as the model, to probe the effect of removal of afferent input on the peptidergic systems. The specific aims are : 1) to determine the effect of baroreceptor denervation on cardiovascular and endocrine function in the conscious rat. The time course of the changes in plasma vasopressin and oxytocin and cardiovascular parameters will be evaluated; 2) to evaluate hormonal and cardiovascular responsiveness in the baroreceptor denervated animal using paradigms in which secretion is stimulated or inhibited; 3) to examine the changes in central responsiveness in the denervated animal using an in vivo microdialysis system for the delivery of stimuli to the paraventricular and supraoptic regions; 4) to determine the mechanisms by which baroreceptor input alters peptide secretion by evaluating the effects of brain stem or hypothalamic lesions and selective neural denervation on endocrine and cardiovascular responses; and 5) to examine the hypothalamic/brain stem interactions by determining the influence of specific lesions of the parvicellular region of the paraventricular nucleus. This investigation will provide information on the interactions between the autonomic nervous system and the endocrine hypothalamus which are critical in cardiovascular fluid/electrolyte balance.