NIDDK/Baylor Biotech Center

描述（由申请人提供）： 用于制造微阵列和新型检测系统的新方法已经 最近在工业和学术环境中开发， 进行新基因发现，分析表达模式， 发展和病理状态。总的来说， 目前通过商业企业提供的产品非常昂贵，而且 超出了小型大学研究实验室预算的范围。的 本提案的总体目标是发展基础设施，使NIDDK能够 资助研究人员获得基因表达微阵列技术。贝勒 医学院（医学院）在发展方面取得了良好的开端， 微阵列技术用于基因表达分析。一个NIDDK/贝勒 生物技术中心将使我们有机会建立最 目前可用于阵列的综合基因组， 组织特异性序列集，将服务于更专门的需要， NIDDK调查员。制作和分析cDNA微阵列的专业知识 存在于贝勒大学。然而，贝勒的设施目前的能力是 有限，不能有效满足研究者的需要。这项建议 将通过提供专门的服务来满足NIDDK调查人员的要求 共享资源核心内的工作人员，他们将推进以下研究目标： 这组调查员。NIDDK/TRAC指导委员会 生物技术中心将协调微阵列生产过程， 分析.该计划的目的包括建立高表达的cDNA微阵列， 密度，能够同时测量基因组的主要部分， 单一反应。我们将开发表示选定子集的自定义数组 的Unigene cDNA文库，对应于在一个表达的基因， 特别是器官/组织，以便加速芯片制备并降低成本。我们 将探索提高微阵列反应灵敏度的策略。 我们将整合生物信息学和生物统计学支持微阵列 分析.我们将开发共享数据、试剂和程序的方法， 贝勒社区和国家，以加强在这一发展的进展 技术.我们将为教师、博士后研究员和 贝勒医学院的学生在个人实验室 由NIDDK以及区域调查员资助。我们会恳求， 选择和支持将受益于微阵列使用的试点项目 基因表达谱最后，我们将建立一个行政结构， 这将支持NIDDK/NIDDK生物技术中心和 NIDDK调查人员将使用该设施。

DESCRIPTION(provided by applicant): New methodologies for fabricating microarrays and novel detection systems have been developed recently in both industrial and academic settings in order to carry out new gene discovery, analysis of expression patterns during development and in pathological states. In general, the technologies that are currently available through the commercial ventures are very expensive, and beyond the reach of small university based research laboratory budgets. The overall goal of this proposal is to develop the infrastructure to enable NIDDK funded investigators to access gene expression microarray technologies. Baylor College of Medicine (BCM) has made an excellent beginning in the development of microarray technologies for gene expression analysis. An NIDDK/Baylor Biotechnology Center would afford us the opportunity to build the most comprehensive set of genes currently available for array and to develop unique, tissue specific sets of sequences that will serve more specialized needs of NIDDK investigators. The expertise to make cDNA microarrays and to analyze them exists at Baylor. However, the current capacity of the facilities at Baylor are limited and cannot effectively meet the needs of investigators. This proposal will address the requirements of NIDDK investigators by providing dedicated staff within shared resource cores who will carry forward the research aims of this group of investigators. The Steering Committee of the NIDDK/BCM Biotechnology Center will coordinate the processes of microarray production and analysis. The aims of this proposal include building cDNA microarrays of high density, enabling simultaneous measurement of a major portion of the genome in a single reaction. We will develop custom arrays representing a selected subset of the Unigene cDNA libraries that correspond to genes expressed in a particular organ/tissue in order to speed chip preparation and reduce cost. We will explore strategies for increasing sensitivity of the microarray reaction. We will integrate bioinformatic and biostatistical support for microarray analysis. We will develop means of sharing data, reagents and procedures within the Baylor community and nationally to enhance progress in this developing technology. We will provide training to faculty, postdoctoral fellows, and students within Baylor College of Medicine in the laboratories of individuals funded by the NIDDK as well as to investigators regionally. We will solicit, select and support pilot projects that would benefit from the use of microarray gene expression profiling. Finally we will develop an administrative structure that will support the activities of the NIDDK/BCM Biotechnology Center and the NIDDK investigators that will utilize this facility.