SUMO Fusion For Genome Wide Protein Analysis

描述（由申请人提供）：从基因组测序项目中发现的大量新型蛋白质为学术界和工业界的科学家创造了巨大的机会。 大规模的联盟和倡议正在进行中，以了解新发现的蛋白质的结构。 不幸的是，蛋白质表达和纯化是一个主要的瓶颈。 细菌通常是重组蛋白表达的首选。LifeSensors Inc.提出了一种新的SUMO融合系统的发展，以提高表达和纯化的各种蛋白质在大肠杆菌。杆菌 SUMO蛋白作为分子伴侣促进包涵体蛋白的表达增强和溶解性。 SUMO融合也被用作快速纯化伴侣蛋白的手柄。 为了检验SUMO的显著性质适用于多种结构多样的蛋白质以及促进不溶性蛋白质的溶解性的假设，申请人提出用多种挑战性蛋白质测试SUMO融合。 为了确定SUMO融合系统相对于现有融合技术的优势，研究人员提出了不同融合系统的定量研究。 开发一种增强蛋白质表达并在基因组水平上提供快速纯化工具的系统将有助于后基因组表达和蛋白质阵列技术。

DESCRIPTION (provided by applicant): A vast number of novel proteins discovered from genome sequencing projects are creating enormous opportunities for scientists in academia and industry. Large-scale consortia and initiatives are under way to understand the structure of newly discovered proteins. Unfortunately, protein expression and purification is a major bottleneck. Bacteria are usually the first order of choice for recombinant protein expression. LifeSensors Inc. proposes the development of a novel SUMO-fusion system to enhance expression and purification of wide variety of proteins in E. coli. The SUMO protein acts as a chaperone to promote enhanced expression as well as solubility of the inclusion body proteins. A SUMO fusion is also used as a handle to rapidly purify partner proteins. To test the hypothesis that the remarkable properties SUMO apply to variety of structurally diverse proteins, as well as promote solubility of insoluble proteins, the applicants propose to test SUMO fusion with variety of challenging proteins. To determine that SUMO fusion system has advantages over the existing fusion technologies, the investigators have proposed a quantitative study with different fusion systems. Development of a system that enhances expression of proteins and offers rapid purification tools at genome level will aid in post-genomic expression and protein array technologies.