STUDY OF INTESTINAL CELL DIFFERENTIATION IN VITRO

该提案的长期目标是定义蜂窝 调控细胞增殖、分化和基因表达的过程 肠子。最基本的前提是细胞周期蛋白依赖的激酶 当肠道干细胞变成激活的隐窝时，抑制物被激活 细胞。P27就是这样一种细胞周期蛋白依赖的激酶抑制物，它可能起作用 不依赖于G1期细胞周期蛋白和CDK。此外，多个冗余基因 除p27外，抑制增殖和分化。因此， 拟议的研究将重点放在抑制p27上，以评估对 分化；阐明jab 1在p27调控中的作用 目的：探讨p27基因的诱导分化特性与其相互关系。 鉴定与p27相互作用的蛋白，并确定 它们在体外和体内的表达；并继续鉴定新的 调节肠道分化的基因。

The long-term goal of the proposal is to define the cellular processes that regulate proliferation, differentiation, and gene expression in the intestine. The fundamental premise is that cyclin-dependent kinase inhibitors are activated when intestinal stem cells become activated crypt cells. One such cyclin-dependent kinase inhibitor is p27 which may act independently from G1 cyclins and cdks. In addition, multiple redundant genes control proliferation and differentiation, apart from p27. Therefore, the proposed studies will focus upon inhibiting p27 to assess effects upon differentiation; to elucidate the role of Jab 1 in the regulation of p27 expression; to correlate differentiation-inducing properties of p27 with a subdomain of the protein; to identify p27 interacting proteins and to determine their expression in vitro and in vivo; and to continue identification of novel genes that regulate intestinal differentiation.