HEMATOPOIETIC GROWTH FACTORS IN A CANINE STEM CELL MODEL

它的目的是对干细胞生理学和 通过实现植入术提高供体可用性和安全性 犬白细胞无移植物抗宿主病(GVHD) 抗原(DLA)半相合和匹配的供体细胞。第二 这一领域的目的是了解生长因子 可以改变全身照射对生产的影响 粒细胞和血小板。具体目标是：(1)界定 犬的造血祖细胞的表型 生长因子动员的外周血及其体外检测 和体内功能；2)建立成功的干细胞移植 基因上Dla相同或相同的犬类文盲中的GVHD DLA-单倍体与细胞供体是否相同；以及3)确定 生长因子或组合对改善骨髓最有效 全身照射的毒性及其作用机制 对这一活动负责。

It is aimed at generating new insights into stem cell physiology and increasing donor availability and safety by achieving engraftment in the absence of graft vs. host disease (GVHD) with dog leukocyte antigen (DLA) haploidentical and matched donor cells. The second area is aimed at understanding the mechanisms whereby growth factors can modify the effects of total body irradiation on production of granulocytes and plateletes. The specific aims are: (1) define the phenotypes of canine hematopoietic progenitors present in the peripheral blood mobilized with growth factors and assay their in vitro and in vivo functions; 2) establish successful stem cell grafts without GVHD in canine litermates which are genotopically DLA-identical or are DLA-haploidentical with the cell donor; and 3) identify which growth factors or combinations are most effective I modifying marrow toxicity of total body irradiation and define the mechanisms responsible for this activity.