Core--Transgenic and Embryonic Stem Cell

癌症通常是一系列复杂的基因改变的结果，这些改变使异常细胞能够经历不受调控的生长。小鼠身上的几种不同的基因改变被证明会增加肿瘤形成的发生率，但即使在这些情况下，也只有生物体内的细胞亚群会产生肿瘤，这表明除了最初的伤害之外，还需要基因改变才能进展为肿瘤形成。我们假设乳腺癌的发生、生长和/或转移都与乳腺上皮细胞和间充质细胞的遗传改变有关。 转基因和胚胎干细胞(TG/ES)核心寻求整合几个研究人员的努力，探索上皮和间质的相互作用和导致乳腺癌发生的作用。TG/ES核心有几个与乳腺肿瘤发生项目相关的具体目标。第一个目标是在组织特异性启动子和可诱导启动子的影响下，开发针对乳腺基质成纤维细胞的表达Cre重组酶的转基因小鼠品系。TG/ES核心还将生产、进口、再衍生、分发和冷冻保存所需的小鼠品系，以执行计划项目赠款中的个别项目。这些包括在乳腺上皮中表达Cre重组酶的小鼠，以及各种携带条件性(loxP侧翼)突变的小鼠，这些突变在项目赠款中的个别项目中有详细说明。

Cancer is often the result of a complex set of genetic alterations that give aberrant cells the ability to undergo deregulated growth. Several different genetic alterations in mice have been shown to increase the incidence of tumor formation, but even in these cases, only subsets of cells within the organism give rise to tumors indicating that genetic changes beyond the initial insult are required to progress to tumor formation. We hypothesize that genetic changes in both the epithelial and mesenchymal compartments of the breast contribute to the initiation, growth and/or metastasis of breast cancer. The Transgenic and Embryonic Stem Cell (TG/ES) Core seeks to integrate the efforts of several investigators working to explore epithelial and stromal interactions and contributions leading to mammary carcinogenesis. The TG/ES Core has several specific goals related to the mammary oncogenesis project. The first of these goals is to develop transgenic mouse strains expressing Cre recombinase targeted to the mammary stromal fibroblasts under the influence of tissue-specific and inducible promoters. The TG/ES Core will also produce, import, rederive, distribute and cryopreserve strains of mice necessary to carry out individual Projects in the Program Project Grant. These include mice expressing Cre recombinase in the mammary epithelium and various mice carrying conditional (LoxP flanked) mutations detailed in individual Projects within the Program Project Grant.