METAPNEUMOVIRUS INFECTIONS OF CHILDREN IN INDIA

描述(由研究人员提供)：人偏肺病毒(HMPV)是一种新发现的急性呼吸道感染(ARI)病毒病原体。没有关于hmpv在印度或任何其他发展中国家的作用的描述。拟议的工作将检验这一假设，即hMPV是印度ARI负担的重要贡献者。印度每年有250万名5岁以下儿童死亡。在这些死亡中，有多达50万人死于阿里。拟议的研究是探索性的，因为没有关于hMPV对印度ARI的贡献的数据。对这些调查的支持将发展在世界上人口第二多的国家研究艾滋病毒的能力。 目的1.在发展中国家印度确定与人类免疫缺陷病毒相关的急性呼吸道感染的比例。在新德里一家大型城市教学医院看到的患有ARI和ALRI的儿童将被纳入这项研究。将描述疾病的临床谱系。鼻咽样本将通过RT-PCR和细胞培养检测hMPV的存在。 目的2.发展印度hMPV的分子检测和基因特征分析技术。将采用实时RT-PCR检测，以提高检测hMPV的灵敏度。将确定hMPV N、F和G基因区域的核苷酸序列，并在印度hMPV和其他国家的病毒之间进行比较。这些数据将有助于定义hMPV的全球流行病学，并为印度的hMPV诊断分析和疫苗开发提供关键信息。 目的3.确定印度一家大型城市教学医院不同年龄段人群中hMPV抗体的流行率。没有关于人乳头状瘤病毒抗体在发展中国家流行的数据。将开发检测hMPV抗体的检测方法，并确定血清阳性率。

DESCRIPTION (provided by investigator): Human metapneumovirus (hMPV) is a newly described viral pathogen of acute respiratory infection (ARI). There are no descriptions of the role of hMPV in India or any other developing country. The proposed work will test the hypothesis that hMPV is an important contributor to the burden of ARI in India. India has 2.5 million deaths per year among children less than 5 years of age. ARI causes as many as 0.5 million of these deaths. The proposed studies are exploratory as there are no data on the contribution of hMPV to ARI in India. Support for these investigations will develop capacity for the study of hMPV in the second most populous country in the world. Aim 1. Determine the proportion of ARI associated with hMPV in a developing country, India. Children with ARI and ALRI seen in a large urban teaching hospital in New Delhi will be enrolled in the study. The clinical spectrum of disease will be described. Nasopharyngeal samples will be tested for the presence of hMPV by RT-PCR and cell culture. Aim 2. Develop techniques for molecular detection and genetic characterization of hMPV from India. Real-time RT-PCR detection will be used to improve the sensitivity of detection of hMPV. Nucleotide sequences for regions of the N, F, and G genes of hMPV will be determined and compared among Indian hMPV and viruses from other countries. These data for will help define the global epidemiology of hMPV and provide critical information relevant to hMPV diagnostic assay and vaccine development for India. Aim 3. Determine the prevalence of antibodies to hMPV among different age groups at a large urban teaching hospital in India. No data are available as to prevalence of antibodies to hPMV in developing countries. Assays for detection of hMPV antibodies will be developed and seroprevalence defined.