Cell culture animal models for SARS-CoV

描述(申请人提供)：2003年春，一场不明病因的疫情在东南亚爆发，并迅速蔓延到其他几个国家。这种名为严重急性呼吸窘迫综合征(SARS)的疾病是由世卫组织和地方当局协调的大规模公共卫生努力确定并控制的。经鉴定，该病为一种新的冠状病毒株，命名为SARS-CoV。为了获得更多关于病毒复制、发病机制和免疫反应的知识，需要建立动物和细胞培养模型。本申请中描述的实验旨在建立1)原代呼吸道上皮细胞感染SARS-CoV的体外组织培养模型，2)使用野生型和免疫缺陷小鼠株建立SARS-CoV感染的小鼠模型，3)未知功能的SARS-CoV ORF结构/功能分析系统。成功完成本申请中概述的目标将为研究SARS-CoV的细胞生物学和发病机制建立系统。

DESCRIPTION (provided by applicant): In the spring of 2003, an outbreak of unknown etiology began in South East Asia and quickly spread to several other countries. The disease, termed severe acute respiratory distress syndrome or SARS, was identified and contained by a massive public health effort orchestrated by the WHO and local authorities. The etiological agent of this disease was identified as a new strain of coronavirus, and given the name SARS-CoV. In order to gain additional knowledge as to the replication, pathogenesis and immune responses induced by the virus, animal and cell culture models are needed. The experiments described in this application are aimed at establishing 1) in vitro tissue culture models for SARS-CoV infection using primary respiratory epithelial cells 2) a mouse model for SARS-CoV infection using wild type as well as immunodeficient mouse strains and 3) structure/function analysis systems for SARS-CoV ORFs of unknown function. Successful completion of the aims outlined in this application will establish systems for investigating the cell biology and pathogenesis of SARS-CoV.