ANTI-CANCER PROTEOLYTIC ANTIBODIES

描述(由申请人提供)：抗体疗法现在对几种疾病很常见。一些独特的抗体含有蛋白酶功能，能够特异性地降解它们的目标抗原。IntegriGen已经克隆了独特的蛋白水解性抗体基因，并将对它们进行改造，以结合和切割具有治疗意义的目标抗原。本项目有三个具体的目标：1)克隆和工程蛋白水解性抗体库，2)合成能够与活性酶特异结合的氟磷酸盐探针，以及3)筛选抗肿瘤模型抗原VEGF的AIM文库和AIM的氟磷酸盐探针，以发现新的抗原特异性蛋白水解性抗体。

DESCRIPTION (provided by applicant): Antibody therapy is now common for several diseases. Some unique antibodies contain a protease function, with the ability to specifically degrade their target antigen. IntegriGen has cloned unique proteolytic antibody genes and will engineer them to bind and cleave therapeutically relevant target antigens. This project has three specific aims: 1) Clone and engineer libraries of proteolytic antibodies, 2) synthesize fluorophosphonate probes capable of specifically binding active proteases, and 3) screen the libraries of aim (1) against the model tumor antigen VEGF and the fluorophosphonate probe of aim (2) to discover new antigen specific proteolytic antibodies.