Modernization of Housing for the Peromyscus Genetic Stock Center, an International Research Resource

Permyscus遗传股票中心住房的现代化 60多年来，南卡罗来纳大学(UofSC)一直维持着Permyscus遗传学股票中心(PGSC)， 一种独特的国际研究资源，由许多来自不同学科的NIH资助的科学家利用，包括 遗传学、表观遗传学、发育生物学和传染病传播。PGSC的使命是 维护各种不同品种、遗传多样性的鹿小鼠(Permyscus属)的群体，并将它们分发给 世界各地的调查人员。今天，我们积极维护六个不同的封闭式无特定病原体(SPF)菌落。这个 PGSC是世界上唯一一个饲养和供应Permyscus老鼠的活体种群。 PGSC最初将Permyscus安置在开放式、不透明的聚丙烯外壳中。在2010年，微隔离器顶部是 使用HEPA过滤的更换站和生物安全柜，以保护人员免受过敏原的影响 并改善群体的健康状况。消除地方性感染以产生SPF级Permyscus小鼠 研究。 2019年，PGSC开始进行试点研究，以评估IVC住房对各种Permyscus物种的影响。 在IVC饲养了两年多的Permyscus小鼠后，我们确定： 1.Permyscus放置在IVC机架上不会产生不良影响 2.Permyscus在IVC机架上保持或提高繁殖效率 3.IVC住房解决了被认为对该物种正常的健康状况。 PGSC希望将整个殖民地改建为IVC住房。我们正在申请资金购买更多的IVC住房 足以将PGSC菌落的剩余部分从静态微隔离器外壳中转化出来。住宅的现代化 这一国际研究资源的条件将： 1.保护人员免受过敏原和潜在病原体的影响 2.提高生物安全，保护动物的SPF状态 3.允许因住房容量增加而扩大殖民地规模，同时保持相同的占地面积 4.显著提高PGSC的运行效率

Modernization of Housing for the Peromyscus Genetic Stock Center For over 60 years, the University of South Carolina (UofSC) has maintained the Peromyscus Genetic Stock Center (PGSC), a unique international research resource utilized by many NIH-funded scientists from a variety of disciplines including genetics, epigenetics, developmental biology, and transmission of infectious disease. The mission of the PGSC is to maintain colonies of various species of outbred, genetically diverse deer mice (genus Peromyscus) and distribute them to investigators around the world. Today, we actively maintain six different closed specific pathogen free (SPF) colonies. The PGSC is the only living stock collection in the world that maintains and supplies Peromyscus mice. The PGSC originally housed Peromyscus in open-top, opaque polypropylene housing. In 2010, microisolator tops were incorporated along the use of HEPA-filtered changing stations and biosafety cabinets to protect personnel from allergens and improve the health status of the colony. Endemic infections were eliminated to generate SPF Peromyscus mice for research. In 2019, the PGSC began conducting pilot studies to evaluate the effects of IVC housing on various Peromyscus species. After housing Peromyscus mice for over two years on IVC housing, we have determined that: 1. Peromyscus exhibit no adverse effects from being housed on IVC racks 2. Peromyscus maintained or improved breeding efficiency on IVC racks 3. IVC housing resolved health conditions that were believed to be normal for the species. The PGSC wishes to convert the entire colony to IVC housing. We are requesting funds to purchase additional IVC housing sufficient to convert the remainder of the PGSC colony from static microisolator housing. Modernization of the housing conditions in this international research resource will: 1. Protect personnel from allergens and potential pathogens 2. Increase biosecurity and protect the SPF status of the animals 3. Allow expansion of colony size due to increased housing capacity while maintaining the same footprint 4. Significantly increase operational efficiency of the PGSC