Alcohol Dependence And Chromosome 11p15.5

为了跟踪染色体11p15.5与酒精依赖的连锁发现，已经创建了两个用于半自动荧光基因分型的短串联重复标记面板，其包含分布在染色体11p15.5的远端20 cM上的15个位点。一个面板（六个位点）已被分型在大约500个精神病学采访的个人从西南美洲印第安部落。第二组（9个基因座）已经优化，分型将很快开始开始。此外，两个编码多态性和一个启动子多态性在DRD4，一个候选基因参与酒精依赖的脆弱性，已被分型，以开发在这个位点的单倍型，远端的短串联重复序列位点在全基因组连锁扫描。使用这些标记构建的单倍型的连锁已经证实了在第二组西南美洲印第安同胞对中的主要连锁发现，表明有助于酒精依赖的基因座在11p15.5区域。

In order to follow-up a linkage finding on chromosome 11p15.5 to alcohol dependence, two short tandem-repeat marker panels for semi-automated fluorescent genotyping containing 15 loci distributed over the distal 20 cM of chromosome 11p15.5 have been created. One panel (six loci) has been typed in approximately 500 psychiatrically-interviewed individuals from a Southwest American Indian tribe. A second panel (nine loci) has been optimized and typing will begin soon. In addition, two coding polymorphisms and one promoter polymorphism at DRD4, a candidate gene for involvement in vulnerability to alcohol dependence, have been typed in order to develop haplotypes at this locus, distal to the short-tandem repeat locus linked in the whole genome linkage scan. Linkage using haplotypes constructed from these markers has confirmed the primary linkage finding in a second panel of Southwest American Indian sib pairs, indicating that a locus contributing to alcohol dependence is in the 11p15.5 region.