An enzyme-based assay for the detection of acetaldehyde-protein adducts

摘要 长期饮酒是世界范围内的一个重大问题，可能会导致成瘾和损害 遍及全身。酒精代谢的第一步是将其转化为乙醛，这是一种高度 活性分子和有毒分子。与蛋白质和DNA形成乙醛加合物是主要的 乙醇毒性的机制。乙醛加合物是酗酒的直接生物标志物。我们打算 建立一种简便、快速的测定乙醛-蛋白质加合物的酶标法来评估酒精 使用和酒精相关的健康后果。

ABSTRACT Chronic alcohol consumption is a significant issue worldwide which can lead to addiction and damage throughout the body. The first step in alcohol metabolism is its conversion into acetaldehyde, which is a highly reactive and toxic molecule. The formation of acetaldehyde adducts with protein and DNA is a primary mechanism of ethanol toxicity. Acetaldehyde adducts are a direct biomarker for alcohol abuse. We intend to develop a facile and rapid enzyme-based assay for measuring acetaldehyde-protein adducts to assess alcohol use and alcohol-related health consequences.