Research Core: Biochemistry and histology/histomorphometry

研究核心将由卡伦·冈德伯格博士指导。该核心将提供直接的骨骼分析 和骨和矿物质代谢的生化测量，以促进皮质醇基础和 翻译研究项目。核心将与耶鲁核心中心的生理学核心合作 肌肉骨骼疾病，并允许必要的服务扩展，以提供 全心全意支持CORT项目。提供的具体服务包括： 1.不脱钙骨组织的固定、包埋、切片和载玻片制备。 2.动物模型骨的特殊染色，包括甲苯胺蓝、von Kossa、Safronin O、 碱性磷酸酶、特异性免疫组织化学定位和原位末端标记法。 3.骨的标准静态和动态参数的测量和解释 组织形态计量学分析。 4.培养中矿化细胞的鉴定。分析将包括茜素红和冯·科萨 矿化培养的染色以及钙和磷含量的直接测量，碱性 磷酸酶和骨钙素。 5.PTH(完整和中分子)，FGF23(完整和C-末端)，25(OH)D，1，25(OH)2D， 血清PINP、人骨钙素、I型胶原C端肽(Crosslaps)和尿矿物质 肌酐将被提供给人体研究。 6.测定小鼠甲状旁腺素、FGF23(完整)、25(OH)D、1，25(OH)2D、小鼠骨钙素、天冬氨酸 I型胶原(Ratlaps)和尿液矿物质将被提供给人类研究。

The Research Core will be directed by Dr. Caren Gundberg. This Core will provide direct skeletal analysis and biochemical measurements of bone and mineral metabolism as to facilitate CORT basic and translational research projects. The Core will partner with the Physiology Core of the Yale Core Center for Musculoskeletal Diseases, and allow for the necessary expansion of services in order to provide the dedicated support of CORT projects. Specific services offered will include: 1. Fixation, embedding, sectioning, and slide preparation of undecalcified skeletal tissue. 2. Specialized staining of bone from animal models, including toluidine blue, von Kossa, Safronin O, alkaline phosphatase as well as specific immunohistochemical localizations and TUNEL assays. 3. Measurement and interpretation of standard static and dynamic parameters of bone histomorphometrical analysis. 4. Assessment of mineralizing cells in culture. Analyses will include both alizarin red and von Kossa staining of mineralizing cultures as well as direct measurement of calcium and phosphorus content, alkaline phosphatase and osteocalcin. 5. Measurement of PTH (intact and mid-molecule), FGF23 (intact and C-terminal), 25(OH)D, 1,25(OH)2D, serum PINP, human osteocalcin, C-telopeptide of type I collagen (Crosslaps) and urinary minerals and creatinine will be provided for human studies. 6. Measurement of mouse PTH, FGF23 (intact), 25(OH)D, and 1,25(OH)2D, mouse osteocalcin, Ctelopeptide of type I collagen (Ratlaps), and urinary minerals will be provided for human studies.