EFFECT OF BROCCOLI SPROUTS ON PHASE II ENZYMES AND RESPIRATORY OXIDATIVE STRE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 具体目标 1.检测西兰花芽通过上调第二相抗氧化酶来预防氧化应激介导的呼吸道炎症的有效性。我们将通过上、下呼吸道细胞因子和8-异前列腺素水平以及GSTM1和NQO1基因的表达来评估上、下呼吸道氧化应激的生物标志物。 2.为了研究GSTM1和NQO1基因的作用，我们已经证明GSTM1和NQO1这两个常见的多态在对柴油废气颗粒(DEP)的佐剂反应中是关键的，在决定对西兰花芽的反应中是关键的。 3.探讨特应性哮喘和轻度间歇性哮喘患者上呼吸道氧化应激对下呼吸道炎症标志物的影响，探讨DEP引起的上、下呼吸道炎症反应与西兰花芽刺激反应之间的相互关系。 4.测试花椰菜芽预防对呼吸道新抗原的原发变态反应的有效性，以DEP为佐剂。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIFIC AIMS 1. To test the effectiveness of broccoli sprouts in preventing oxidative-stress mediated respiratory inflammation through the upregulation of Phase II antioxidant enzymes. We will assess biomarkers of oxidative stress in the upper and lower airway as measured by upper and lower airway cytokine and 8-isoprostane levels and by GSTM1 and NQO1 gene expression. 2. To examine the role of GSTM1 and NQO1 genotypes, common polymorphisms we have shown to be critical in the adjuvant response to diesel exhaust particles (DEP), in determining responsiveness to broccoli sprouts. 3. To investigate the effects of upper airway oxidative stress on inflammatory markers of the lower airway in subjects with atopy and mild intermittent asthma, thereby testing the interrelationship between upper and lower airway inflammation in response to DEP and response to broccoli sprouts. 4. To test the effectiveness of broccoli sprouts in preventing primary allergic sensitization to a respiratory neaoantigen administered with DEP as an adjuvant.