INTRARENAL OXYGENATION AND OXIDANT STRESS IN PATIENTS WITH CKD AND CAN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项为期两年的前瞻性研究使用了一种名为血氧水平依赖磁共振成像(BOLD MRL)的非侵入性成像技术。我们计划确定(1)慢性肾脏疾病(CKD)和慢性移植物肾病(CAN)是否与肾脏氧合不良有关(2)氧合不良是否会导致CKD氧化应激(OS)增加，以及(3)血管紧张素受体阻滞剂氯沙坦是否在肾脏具有抗氧化特性。这项研究将包括一组健康受试者和两组患者(CKD和CAN)。受试者将被安排进行两次检查(相隔24个月)，在口服50毫克氯沙坦之前和之后采集血液和尿样。研究人员还将在氯沙坦治疗前后对其肾脏进行大胆的核磁共振检查。将对尿样和血样进行检查，以测量肾功能和OS的各种标志。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This two-year prospective study uses a noninvasive imaging technique called blood oxygenation level-dependent magnetic resonance imaging (BOLD MRl). We plan to determine (1) whether chronic kidney disease (CKD) and chronic allograft nephropathy (CAN) are associated with poor kidney oxygenation (2) whether poor oxygenation results in increased oxidant stress (OS) in CKD and CAN (3) whether losartan, an angiotensin receptor blocker, has anti-oxidant properties in the kidney. The study will include a group of healthy subjects and two groups of patients (CKD and CAN). Subjects will be scheduled for two visits (24 months apart), where blood and urine samples will be collected before and after a 50 mg oral dose of losartan. Individuals will also undergo BOLD MRI testing of their kidney before and after losartan. Urine and blood samples will be examined to measure kidney function and various markers of OS.