COBRE: UVM MED: CORE C: CELLULAR & MOLECULAR

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 佛蒙特大学神经科学COBRE细胞和分子生物学核心设施为希望在研究中利用细胞和分子生物学的研究人员提供设备和专业知识，但他们可能没有必要的经验或设备。该设施包括以下主要设备：Ciphergen SELDI-TOF质谱仪、Zeiss Palm激光显微切割系统、Applied Biosystems 7500 FAST定量PCR系统、Odyssey红外成像仪、Neuroleucida神经解剖分析系统、BioRad 2-D凝胶系统和Beckman Ultracentheges。该设施还配备了标准的分子生物学实验室设备，包括：3台Techne热循环仪，1台BioRad梯度循环仪，分光光度计，光度计，细胞培养罩，培养箱和显微镜，Zeiss荧光显微镜，低速离心机，振荡培养箱和其他几个培养箱。这一设备用于各种各样的项目，在报告所述期间，用户群为32名教师、12名博士后、6名研究生和5名本科生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Vermont Neuroscience COBRE Cell and Molecular Biology Core facility provides equipment and expertise for researchers wishing to utilize cell and molecular biology in their research, but who may not have the experience or equipment necessary. The facility contains the following major pieces of equipment: Ciphergen SELDI-TOF Mass Spectrometer, Zeiss Palm Laser Microdissection System, Applied Biosystems 7500FAST Quantitative PCR System, Odyssey Infrared Imager, Neuroleucida Neuroanatomical Analysis System, BioRad 2-D Gel System and Beckman Ultracentrifuges. The facility is also fully equipped with the standard molecular biology laboratory equipment including: 3 Techne thermal cyclers, 1 BioRad gradient cycler, spectrophotometer, luminometer, cell culture hood, incubators and microscopes, Zeiss fluorescence microscope, low-speed centrifuges, shaking incubator, and several other incubators. This equipment is used on a wide variety of projects, with a user base during the reporting period of 32 faculty, 12 postdoctoral associates, 6 graduate students and 5 undergraduate students.