CRYSTAL STRUCTURES OF HUMAN ANNEXIN A2 IN COMPLEX WITH PHOSPHATIDYL-INOSITOL-4,5

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 膜联蛋白A2是钙和磷脂结合蛋白家族的成员，称为膜联蛋白。由于其结合磷脂膜和细胞骨架的能力，该蛋白似乎在膜相关事件中发挥作用，如内吞作用、胞吐作用、膜/膜和膜/细胞骨架连接。最近已经表明，膜联蛋白A2特异性地与含有磷脂酰肌醇-4，5-二磷酸的细胞膜部分结合。这种相互作用对磷脂酰肌醇-4，5-二磷酸具有特异性，而对其他磷酸肌醇没有特异性。该复合物的解离常数在微摩尔范围内。我们纯化了重组人膜联蛋白A2，并在L-alpha-磷脂酰肌醇-4，5-二磷酸（仅脂质的头基）存在下使其结晶。我们想确定蛋白质复合物的高分辨率结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Annexin A2 is a member of the calcium- and phospholipid binding protein family called annexins. Due to its ability to bind to phospholipid membranes and the cytoskeleton, the protein seems to play a role in membrane-related events such as endocytosis, exocytosis, membrane/membrane and membrane/cytoskeleton linkage. It has been shown recently that annexin A2 specifically associates with parts of the cell membrane containing phosphatidyl-inosiol-4,5-biphosphate. The interaction is specific for phosphatidyl-inositol-4,5-biphosphate and not for other phosphoinositides. The dissociation constant for this complex is in the micromolar range. We have purified recombinant human annexin A2 and crystallized it in the presence of L-alpha-phosphatidylinositol-4,5-diphosphate (the headgroup of the lipid only). We would like to determine the high resolution structure of the protein complex.