TOMOGRAPHY OF SKELETAL MUSCLE TRIADIC JUNCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 摘要： 目的是重建骨骼肌中两个钙通道(膜/T管DHPR的L型钙通道)与肌浆网钙释放通道之间的特异性联系。这可以通过对完整的肌肉纤维中的SR/T小管连接进行断层扫描来完成，并且需要奥尔巴尼资源中心提供的特殊仪器：高压快速冰冻设备、用于冰冻切片的超微切割机和高倾角的中高压冷冻电子显微镜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: The aim is to reconstruct the specific association between two calcium channels in skeletal muscle (the L type calcium channel of DHPR of the surface membrane/T tubules) and the calcium release channel of the SR. This can be done by tomography of the SR/T tubule junctions in an intact muscle fiber, and requires specific instrumentation available at the Albany Resource: A high pressure rapid freezing apparatus, an ultramicrotome for frozen sections, and intermediate high voltage cryo-electron microscope with high tilt angle.