RAMAN SCATTERING STUDIES OF BACTERIORHODOPSIN IMMOBILIZED ON CARBON NANOTUBES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物纳米技术试图利用蛋白质光刻的方法，将蛋白质功能固定在合适的锚定平台上，以用于技术应用。光感受器膜蛋白，如细菌视紫红质(BR)，它是光激活的，可以通过合理的定点突变来调节，以增加其热稳定性和光化学敏感性。这些蛋白质已经成为技术应用中的主要候选者。与其他基于蛋白质的生物分子器件相比，与碳纳米管偶联的蛋白质具有更高的技术应用潜力。单壁碳纳米管的纳米尺寸和电子性质使其成为锚定蛋白质用于生化传感的理想底物。共振拉曼光谱和表面增强拉曼光谱将用于表征细菌视紫红质-碳纳米管复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bionanotechnology seeks to harness protein functions for technological applications by immobilizing them on suitable anchoring platforms using methods of protein lithography. Photoreceptor membrane proteins, such as bacteriorhodopsin (bR), which are light-activated and can be tuned through rational site-directed mutagenesis to increase their thermal stability, photochemical sensitivity. These proteins have emerged as leading candidates in technological applications. Proteins coupled to CNT have a higher potential for technological applications than other protein-based biomolecular devices. The nano-dimensions of single walled carbon nanotubes and their electronic properties make them an ideal substrate candidate for anchoring the proteins for biochemical sensing. Resonance Raman and surfaced-enahanced Raman spectroscopy will be used to characterize the bateriorhodopsin-carbon nanotube complexes.