A MULTICOMPONENT COMPLEX CONTROLS ENVIRONMENTAL SIGNALING IN BACILLUS SUBTILIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一般的应激反应有助于细菌在自然环境、食物和病原体的相互作用中生存。枯草杆菌的一般应激信号途径与相关的人类病原体金黄色葡萄球菌、单核细胞增生性李斯特菌、炭疽杆菌和结核分枝杆菌的途径非常相似。我们已经分离到一个与环境胁迫信号有关的700 kDa枯草杆菌复合体。首先通过亲和层析从粗提物中纯化了该络合物，然后根据大小进行了纯化。根据考马斯染色的凝胶判断，峰部分包含至少20种蛋白质，其中5种是使用针对已知参与该途径的蛋白质的抗体鉴定的。一些未知蛋白可能是污染物或伴侣蛋白，而另一些可能是压力敏感蛋白。一旦确定了该复合体中的一些未知蛋白质，我们就可以破坏它们的结构基因，以了解这些产物是否确实具有信号作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general stress response contributes to bacterial survival in the natural environment, in food, and in pathogenic interactions. The general stress signaling pathway in B. subtilis closely resembles the pathways in the related human pathogens Staphylococcus aureus, Listeria monocytogenes, Bacillus anthracis, and Mycobacterium tuberculosis. We have isolated a 700 kDa B. subtilis complex involved in environmental stress signaling. The complex was purified from crude extracts first by affinity chromatograpy and then by size. Judging by a coomassie-stained gel, the peak fractions contain at least 20 proteins, five of which were identified using antibodies specific for proteins known to be involved in the pathway. Some of the unknown proteins may be contaminants or chaperones, whereas other may be stress sensing proteins. Once some of the unknown proteins in the complex are identified, we can disrupt their structural genes to learn if the products do in fact have signaling role.