COPPER SPATIAL DISTRIBUTION, CONCENTRATION & STATUS IN NORMAL & TUMOR TISSUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在以前的实验中，我们已经证明了DSF和CQ可以在溶液中与铜作用形成一种新的化合物。CQ-铜和DSF-铜的组合可作为毒性最低的抗癌药物。为了研究铜在正常组织和肿瘤组织中的分布，我们对小鼠乳腺和小鼠前列腺组织的正常组织和肿瘤组织进行了元素荧光图谱的研究。我们还在处理后的组织中进行了定位实验，以考察上述化合物对体内铜分布的影响。使用的样本是乳腺癌和前列腺癌组织。我们的数据表明，铜在正常组织中含量很低，在肿瘤组织中含量较高。我们还测量了铜、锌和铁在两组样品中分布的相关性。结果表明，这些化合物在肿瘤组织样本中具有显著的活性。更多的样本正在准备中，以调查这些药物在肿瘤发展的不同阶段的效果。实验条件的优化也在进行中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In a previous experiment, we have demonstrated that DSF and CQ can interact with Cu in solution forming a new compound. The combined CQ-Cu and DSF-Cu compounds can be used as anticancer drugs with minimum toxicity levels. To investigate the Cu distribution in normal and tumor tissues we performed elemental fluorescence mapping on normal and tumor tissue from mouse breast and mouse prostate tissue. We also performed mapping experiment in treated tissues to investigate the effect of the mentioned compounds in the Cu distribution in vivo. The samples used were breast cancer and prostate cancer tissue. Our data indicate that Cu is found in very low amount in normal tissue and in higher levels in tumor tissue. We have also measured the correlation of Cu, Zn and Fe distributions in both sets of samples. The results indicate a significant activity of the compounds at the tumor tissue samples. More samples are under preparation to investigate the effect of the drugs at different stages of the tumor development. Optimization of the experimental conditions is also under development.