Facility Core D--Cell Imaging & Analysis

组织和分离细胞的成像是用于确定生物化学和分子生物学特性的关键工具。 涉及组织、细胞和亚细胞器的表型和功能变化的机制 是由接触异生物质引起的影像学、生物化学和分子生物学的最新进展 生物学，导致了成像技术的升级，并在生物医学科学中的使用超过了 过去几年。荧光探针、绿色荧光蛋白（GFP）、共聚焦和荧光标记的开发 双光子光学和光电探测器已经允许单分子的可视化， 监测活细胞中的分子相互作用，并增强对复杂组织成像的能力。的 开发最先进的成像技术所需的复杂性、时间和成本使得 个体研究者无法独立获得这些重要的工具和方法， 生物医学研究因此，需要有一个有组织的专家小组， 在成像技术，以获得仪器，掌握应用程序，并协助个人 研究人员使用该技术来回答特定的研究问题。 细胞成像和分析设施的长期目标是提供最先进的 在流式细胞术和细胞分选、组织病理学和显微镜方面的仪器和专业知识。我们 目标是开发基于荧光的技术，以满足中心当前和未来的需求 investigators.我们的理由是，获得最先进的荧光技术将使中心 研究人员在细胞水平上确定异生素作用的具体机制。被激励 该设施的互动工作人员提供成像和分析互补领域的专业知识。博士 Kerkvliet和Oughton女士拥有超过40年的流式细胞术经验， 研究生产力的杰出记录。格林伍德博士和弗雷利女士最近建立了 共聚焦和宽视场显微镜系统，出版研究所产生的发展， 光漂白后的荧光恢复和荧光共振能量转移显微术。博士 Lohr是一名经过认证的兽医病理学家，与中心有着重要的合作互动 最近的出版物表明，费舍尔女士在这方面有20多年的经验， 组织学、图像分析和电子显微镜。最后，我们与Molecular Probes/Invitrogen提供试剂和专业知识，使我们特别有能力 实现我们的目标。

Imaging of tissues and isolated cells is a critical tool for determining the biochemical and molecular mechanisms involved in phenotypic and functional changes in tissues, cells, and subcellular organelles induced by exposure to xenobiotics. Recent advances in imaging, biological chemistry, and molecular biology, have led to an escalation in imaging technologies and use in the biomedical sciences over the last several years. Development of fluorescent probes, green fluorescent protein (GFP), confocal and two-photon optics, and photoelectronic detectors, have allowed the visualization of single molecules, monitoring of molecular interactions in live cells, and enhanced ability to image complex tissues. The complexity, time, and cost required for developing state-of-the-art imaging technologies makes it unfeasible for individual investigators to independently obtain these important tools and approaches for biomedical research. Therefore, the need exists for an organized group of specialists with the expertise in imaging technologies to acquire the instrumentation, master the applications, and assist individual investigators in using the technology to answer specific research questions. The long-term goal of the Cell Imaging and Analysis Facility is to provide access to state-of-the-art instrumentation and expertise in flow cytometry and cell sorting, histopathology, and microscopy. Our objective is to develop fluorescence-based technologies to meet the current and future needs of Center investigators. Our rationale is that access to state-of-the-art fluorescence technologies will allow Center investigators to determine specific mechanisms of xenobiotic action at the cellular level. The motivated and interactive staff of the facility provides expertise in complementary areas of imaging and analysis. Dr. Kerkvliet and Ms. Oughton have over 40 years combined experience in flow cytometry with an outstanding record of research productivity. Dr. Greenwood and Ms. Fraley have recently setup the confocal and widefield microscopy systems, publishing studies resulting from the development of fluorescence recovery after photobleaching and fluorescence resonance energy transfer microscopy. Dr. Lohr is a certified Veterinary Pathologist with important collaborative interactions with Center investigators demonstrated by recent publications and Ms. Fischer has over 20 years of experience in histology, image analysis, and electron microscopy. Lastly, our collaboration with Molecular Probes/lnvitrogen, providing access to reagents and expertise, makes us especially well-positioned to accomplish our objective.