THE USE OF URINARY PCR TEST TO DETECT REJECTION IN KIDNEY TRANSPLANT PATIENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 尿液聚合酶链式反应测试已经被开发出来，以帮助检测肾移植过程中的排斥反应。对移植受者的尿样进行分析，以寻找在排斥反应中已知表达的特定基因，即颗粒酶B和穿孔素。此外，患者还会接受移植肾的“常规”活检，目的是显示聚合酶链式反应结果与肾脏病理之间的相关性，当其他测试为阴性时，也可以用来检测排斥反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The urinary PCR test has been developed to help detect rejection in kidney transplant procedures. A urine sample from a transplant recipient is analyzed for specific genes that are known to be expressed during rejection, namely Granzyme B and Perforin. In addition, patients undergo "protocol" biopsies of the transplanted kidney which aims to show the correlation between the PCR results and the pathology of the kidney, and may also serve to detect rejection when other tests are negative.