COBRE: U NEL: CORE B: SPECTROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该设施为本提案中的研究提供服务支持。 许多关于氧化还原反应和氧化还原中心之间的相互作用的重要信息是通过光谱方法获得的。 电子顺磁共振（EPR）谱是确定顺磁氧化还原中心电子结构的关键仪器之一。 该信息补充了大分子的紫外可见光谱和荧光光谱。 圆二色性（CD）光谱用于确定蛋白质的一些宏观性质，例如大分子之间以及大分子与底物或抑制剂之间的整体构象，其控制反应速率。 光谱学核心的主要任务是： " 为RBC研究人员、非RBC研究人员和外部研究人员提供最先进的光谱仪器服务; " 帮助促进内布拉斯加大学系统内外RBC研究人员和非RBC研究人员之间的密切合作; " 为研究生、博士后及其他感兴趣的人员提供高质量的光谱仪器培训。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This facility provides service support for the research in this proposal. Much of the important information about redox reactions and interactions between redox centers is obtained by spectroscopic methods. Electron paramagnetic resonance (EPR) spectroscopy is one of the key instruments used to determine the electronic structure of paramagnetic redox centers. This information complements UV-visible and fluorescence spectra of the macromolecule. Circular dichroism (CD) spectroscopy is used to determine some macroscopic properties of a protein, such as overall conformation between macromolecules and between a macromolecule and a substrate or inhibitor governs the rate of reactions. The primary missions of the Spectroscopy Core are: " To provide state-of-the-art spectroscopy instrumentation services to RBC researchers, non-RBC researchers, and outside investigators; " To help facilitate strong collaborations between RBC researchers and non-RBC researchers within and outside the University of Nebraska system; and " To provide high quality spectroscopy instrumentation training to graduate students, postdoctoral students, and other interested personnel.