BIOSYNTHETIC MECHANISMS FOR FUNGAL POLYKETIDES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 许多重要的聚酮类天然产物都是由丝状真菌产生的，例如由镰刀菌产生的真菌毒素伏马菌素和由土曲霉产生的降胆固醇药物洛伐他汀(MervorTM)。我们正在使用遗传和生化方法来了解真菌合成这些复杂代谢物的机制。生物合成基因被特定地敲除并在功能上互补，以定义基因的作用。生物合成酶在异种宿主中产生，如大肠杆菌和烘焙酵母，以确定这些酶催化的反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many important polyketide natural products are produced by filamentous fungi, such as the mycotoxin fumonisins produced by Fusarium verticillioides and the cholesterol-lowering drug lovastatin (MervacorTM) by Aspergillus terreus. We are using genetic and biochemical approaches to understand the mechanism by which the fungi synthesize these complex metabolites. The biosynthetic genes are specifically knocked out and functionally complemented to define the role of the genes. The biosynthetic enzymes are produced in heterologous hosts, such as E. coli and bakers yeast, to determine the reactions catalyzed by these enzymes.