P4: ER TO GOLGI T & VSMC

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目的是确定在原代培养的新生大鼠心室肌细胞和血管平滑肌细胞中，血管紧张素II 1型受体（AT 1 R）和β 2肾上腺素能受体（AR）的ER-高尔基体转运改变的作用。有三个具体目标。第一个目的是确定Rab 1和其他囊泡转运调节因子（Sar 1，ARF 1，Rab 2和Rab 6）在内质网到高尔基体转运中的作用。第二个目的是确定是否在ER到高尔基体运输的改变，作为操纵Rab 1功能的结果，参与受体信号传导。第三个目的是确定Rab 1对蛋白表达谱的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this project is to determine the role of altered ER-to-Golgi trafficking of angiotensin II type 1 receptor (AT1R) and beta 2 adrenergic receptor (AR) in primary cultures of neonatal rat ventricular myocytes and vascular smooth muscle cells. There are three specific aims. First aim is to define the role of Rab1 and other vesicular transport regulators (Sar1, ARF1, Rab2 and Rab6) in the endoplasmic reticulum-to-Golgi transport. Second aim is to determine if alterations in the ER-to-Golgi transport, as a consequence of manipulating Rab1 function, is involved in receptor signaling. Third Aim is to determine the effect of Rab1 on protein expression profile.