COBRE: UOK HSC: P 3: GENETIC & BIOCHEMICAL STUDIES OF STREP MUTANS BIOFILM FORMA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 描述IrvA在应激反应调节中的作用。 IrvA是一种新的变形链球菌应激诱导调节因子。 它似乎是用于在严重应激情况下抑制毒力基因表达的系统的核心组成部分。 为了进一步表征该系统，我们计划确定IrvA直接控制下的基因和负责irvA诱导的途径。 变形链球菌hdrRM操纵子对感受态基因调控的遗传和生化研究。 最近，我们确定了一个双基因操纵子，是由极高的细胞密度的条件下诱导的，是几个密度依赖性毒力因子，如自然能力的功能所需的。 我们计划确定感受态基因调控的机制。 此外，这两个基因似乎形成了一种新型的调控系统。 因此，我们计划研究其信号转导机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Characterize the role of IrvA in stress response regulation. IrvA is a novel, stress induced regulator of Streptococcus mutans. It appears to be a central component of a system used to suppress virulence gene expression under situations of severe stress. To further characterize this system, we plan to determine the genes under the direct control of IrvA and the pathway responsible for irvA induction. Genetic and biochemical studies of competence gene regulation by the hdrRM operon of Streptococcus mutans. Recently, we identified a two-gene operon that is induced by conditions of extremely high cell density and is required for the functioning of several density-dependent virulence factors, such as natural competence. We plan to determine the mechanism of competence gene regulation. In addition, the two genes appear to form a novel type of regulatory system. Therefore, we plan to investigate its mechanism of signal transduction.