Egg to Embryo: Gene Regulatory Circuitry in Development

本次续签计划项目申请的主要目标仍与其 前人在《早期胚胎调控信息流的分子基础》中提出 然而，由于我们在这个项目上的工作，在很大程度上， 这些单词的含义已被转换，此续订应用程序专注于 我们现在面临的机会是解决胚胎中的调节相互作用流问题 通过确定指导其发展的基因调控网络(GRN)。GRN主要由基因组顺式调控序列控制的相互作用组成，这些顺式调控序列控制着编码转录因子和一些信号成分的基因。初始输入是母性因子，每个空间域的最终输出是编码分化蛋白的基因的激活。在该计划的Davidson组件中，对紫海胆胚胎发生的GRN分析将扩展到整个胚胎，几乎整个胚胎发生(从受精后不久到大约2.5天)。Fraser组件专注于使用海胆和海鞘胚胎对GRN的活动进行成像的新方法。在Levine成分中，海胆胚胎的内胚层GRN的关键联系将在海囊类胚胎中寻找，并将研究在脊索动物和棘皮动物之间共享的胚胎发生的这一基本过程中存在基本顺式调控密码的可能性。行政科将监督财务、材料和智力方面的管理、预算和实验室间协调。还需要一个与目前的研究核心服务设施类似但不完全相同的研究核心服务设施，其基本作用是向研究的三个组成部分提供胚胎、卵子和紫锥虫的核提取物。

The major objective of this Renewal Program Project Application remains as stated in its predecessor, to determine "the molecular basis of regulatory information flow in early embryonic development." However, to a significant extent as a result of our work on this Program, the meaning of these words has been transformed, and this Renewal Application is focused on the opportunity now confronting us to solve the problem of regulatory interaction flow in the embryo by determining the gene regulatory network (GRN) that directs its development. The GRN consists largely of interactions mandated by genomic cis-regulatory sequences controlling genes that encode transcription factors and some signaling components. The initial inputs are maternal factors, and the terminal output in each spatial domain is activation of genes encoding differentiation proteins. In the Davidson Component of the Program, GRN analysis of embryogenesis in the sea urchin, Strongylocentrotus purpuratus will be extended to the whole of the embryo, and almost the whole of embryogenesis (from shortly after fertilization to about 2.5 days). The Fraser Component is focused on novel approaches to imaging the activity of GRNs, using sea urchin and ascidian embryos. In the Levine Component key linkages of the endomesoderm GRN of the sea urchin embryo will be sought in the ascidian embryo, and the possibility will be examined that there is a basal cis-regulatory code for this fundamental process in embryogenesis shared between chordates and echinoderms. The Administrative section will oversee management, budget, and interlaboratory coordination with respect to financial, material and intellectual matters. A Research Core Service Facility similar but not identical to the current one is also requested, the basic role of which is to provide embryos, eggs and nuclear extracts of S. purpuratus to the three Research Components.