COBRE: UVM MED: CORE C: CELLULAR & MOLECULAR

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 佛蒙特大学神经科学COBRE细胞和分子生物学核心设施为希望在研究中利用细胞和分子生物学但可能没有必要经验或设备的研究人员提供设备和专业知识。该设施包括以下主要设备：Ciphergen SELDI-TOF质谱仪、Zeiss Palm激光显微切割系统、2台Applied Biosystems 7500 FAST定量PCR系统、Odyssey红外成像仪、BioRad Calligrapher阵列打印机、Neuroleucida神经解剖分析系统、BioRad 2-D凝胶系统和Beckman Ultracentruges。该设施还配备了标准的分子生物学实验室设备，包括三台Techne热循环仪，一台BioRad梯度循环仪，一台Nanodrop分光光度计，一台光度计和细胞培养罩，以及各种培养箱和显微镜：蔡司荧光显微镜，低速离心机，振荡培养箱和其他几个培养箱。各种项目和研究人员都使用这一设备，在本报告所述期间，用户群有40名教员、27名工作人员、14名博士后、25名研究生和5名本科生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Vermont Neuroscience COBRE Cell and Molecular Biology Core facility provides equipment and expertise for researchers who wish to utilize cell and molecular biology in their research but may not have the necessary experience or equipment. The facility contains the following major pieces of equipment: Ciphergen SELDI-TOF Mass Spectrometer, Zeiss Palm Laser Microdissection System, 2 Applied Biosystems 7500FAST Quantitative PCR Systems, Odyssey Infrared Imager, BioRad Calligrapher Array Printer, Neuroleucida Neuroanatomical Analysis System, BioRad 2-D Gel System and Beckman Ultracentrifuges. The facility is also fully equipped with standard molecular biology laboratory equipment including three Techne thermal cyclers, one BioRad gradient cycler, one Nanodrop spectrophotometer, a luminometer and cell culture hood, as well an as assortment of incubators and microscopes: a Zeiss fluorescence microscope, low-speed centrifuges, a shaking incubator, and several other incubators. This equipment is employed by a wide variety of projects and researchers, with a user base during the reporting period of 40 faculty, 27 staff members, 14 postdoctoral associates, 25 graduate students and 5 undergraduate students.