ISOLATION & CHARACTERIZATION OF CARDIOACTIVE COMPOUNDS IN HI BOX JELLYFISH VENOM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 夏威夷箱水母（Carybdea阿拉塔）属于最臭名昭著的有毒水母类，立方水母，这是常见的热带和亚热带沃茨从夏威夷到澳大利亚。 立方虫叮咬是非常痛苦的，并导致潜在的危及生命的伊鲁坎吉综合征，这是一系列的全身体征和症状，包括心肺窘迫。 急诊室患者的回顾性临床分析表明，Irukandji综合征发生在约5%的当地箱水母蜇伤受害者。 尽管如此，这种综合征的确切生化基础尚不清楚。 在夏威夷、澳大利亚和印度尼西亚，目前的治疗仅限于对症治疗。 这项研究提案是由我们最近发现的小分子引起的，这些小分子在化学上类似于某些昆虫毒素，它们会引起多种生理效应，包括对小龙虾和小鼠的深刻心脏影响。该项目的具体目标是集中精力纯化、鉴定和生理学表征这些具有高度心脏活性的化合物。 总体目标是为这些化合物的作用机制提供急需的见解，并为更好的临床管理提供基础。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hawaiian box jellyfish (Carybdea alata) belong to the most notoriously toxic class of jellyfish, the cubozoans, which are commonly found in tropical and subtropical waters from Hawaii to Australia. Cubozoan stings are extremely painful and result in potentially life-threatening Irukandji syndrome, which presents as a constellation of systemic signs and symptoms, including cardiopulmonary distress. A retrospective clinical analysis of emergency room patients demonstrates that Irukandji syndrome occurs in about 5% of local box jellyfish sting victims. Still, the precise biochemical basis for this syndrome is unclear. In Hawaii, Australia and Indonesia, current therapy is limited to symptomatic care. This research proposal is prompted by our recent discovery of small molecules, chemically similarly to certain insect toxins, which induce multiple physiological effects including profound cardiac effects in both crayfish and mice. The specific aims of this project are to conduct focused efforts to purify, identify and physiologically characterize these highly cardioactive compounds. The overall goal is to provide much-needed insights into the mechanisms of action of these compounds and provide the basis for better clinical management.