Clinical Applications of Molecular Biology in Bladder Cancer

高通量线粒体DNA分析鉴定与肿瘤相关的特异性遗传改变 膀胱癌拟议的工作旨在探讨几个临床问题，膀胱 基于检测原发性肿瘤和尿液中的这些遗传变化的癌症管理。 本课题的具体目标是：（1）确定线粒体DNA的敏感性和特异性 用于检测复发性浅表性膀胱癌的面板，（2）初步探索 线粒体DNA改变的特定模式是否与疾病风险增加相关 浅表性和浸润性癌症的复发或进展。这些具体目标将是 通过前瞻性分析配对的肿瘤和尿液标本， 接受治疗的浅表性和浸润性膀胱癌患者， 约翰霍普金斯医院和湾景约翰霍普金斯医疗中心。分析将是 使用项目#1和2中开发的分子方法和技术进行。更大的目标 本项目的目的是为今后的线粒体DNA分析研究奠定实践基础， 监测和建立不同阶段膀胱癌患者的风险概况 癌

High throughput mitochondrial DNA analysis identifies tumor-specific genetic alterations associated with bladder cancer. The proposed work seeks to explore several clinical issues related to bladder cancer management based on the detection of these genetic changes in primary tumors and urine. IThe specific aims of this project are: (1) to define the sensitivity and specificity of mitochondrial DNA panels for the detection of recurrent superficial bladder cancers and, (2) to preliminarily explore whether specific patterns of mitochondrial DNA alterations correlate with increased risk of disease recurrence or progression in both superficial and invasive cancers. These specific aims will be accomplished through prospective analysis of paired tumor and urine specimens obtained from patients with superficial and invasive bladder cancers undergoing therapy with curative intent at The Johns Hopkins Hospital and The Bayview Johns Hopkins Medical Center. The analyses will be performed using molecular methods and technology developed in projects #1 and 2. The larger goal of this project is to lay a practical foundation for future studies of mitochondrial DNA analysis for monitoring and for the establishment of risk profiles for patients with different stages of bladder cancer.