CORE 1 TRP2: PROTEASE ACTIVITY IMAGING TECHNOLOGY (PAIT)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 技术研究项目2，蛋白酶活性成像技术(PAIT)，将开发 细胞蛋白分解的三维和四维成像技术。两个独立的和 将采取互补的办法。首先，高通量底物噬菌体展示系统将 被设计用来为绝大多数编码的蛋白酶识别高度选择性的指示底物 在基因组中。第二，将应用化学策略开发基于活动的探针， 酶激活的荧光报告。这两套信标都将在生化和 细胞模型，最终将被用于在细胞水平上对蛋白质分解进行成像

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technical Research Project 2, Protease Activity Imaging Technology (PAIT), will develop technology for imaging of cellular proteolysis in three and four dimensions. Two independent and complementary approaches will be taken. First, a high throughput substrate phage display system will be devised to identify highly selective indicator substrates for the vast majority of proteases encoded in the genome. Second, a chemical strategy will be applied to develop activity-based probes with an enzymatically activated fluorescent reporter. Both sets of beacons will be tested in biochemical and cellular models and will ultimately be used to image proteolysis at the cellular level