CORE--Biomarker

临床和转化研究最困难的部分之一是监测疾病的任务 活动和发展对疾病机制的见解。一种行之有效的寻址方法 这些问题是来自人类和动物样本的生物标志物的测量。这个核心的目标是 提供本SCCOR各项目样品中生物标志物的测量 程序.为了实现这一目标，我们将为这些单独的项目提供统一的资源， 大规模测量血浆、血清和尿液中的生物标志物。我们还将使用组织培养 开发将患者样品暴露于培养细胞的报告基因测定的方法， 测量诱发反应。我们亦会集中及组织贮存来自 因此，样品可随时用于任何新的或新兴的生物标志物的测定， 在这个项目的实施过程中。在这种情况下测量生物标志物的主要手段是 核心单元将是市售酶联免疫吸附测定（ELISA）或 放射免疫测定（RIA）。由于某些检测试剂盒可能无法在市场上买到，因此该核心还将 使用市售抗体生成内部ELISA和RIA方法。该核心单位将 生成涵盖SCCOR计划所有项目的数据，从而为以下方面提供共同基础： 动物模型和人类疾病之间的比较。

One of the most difficult components of clinical and translational research is the task of monitoring disease activity and developing insights into the mechanisms of disease. One well established method of addressing these issues is the measurement of biomarkers from human and animal specimens. The goal of this Core is to provide for the measurement of biomarkers in samples derived from the individual projects of this SCCOR program. In order to accomplish this goal, we will offer these individual projects a uniform resource for the large-scale measurement of biomarkers in plasma, serum, and urine. We will also use tissue culture methods to develop reporter assays that will employ the exposure of patient samples to cultured cells and measuring the evoked response. We will also centralize and organize the storage of samples from the individual projects so that samples are readily available for assay with any new or emerging biomarkers that become available during the course of this project. The principal means of measuring biomarkers in this core unit will be commercially available enzyme-linked immunoabsorbant assays (ELISAs) or radioimmunoassays (RIAs). Because some assays may not be commercially available, this core will also generate in house ELISA and RIA methods using commercially-available antibodies. This core unit will generate data that spans all projects of the SCCOR program and thus, should provide a common basis for comparison between animal models and human disease.