Characterization of the Sigma-2 receptor

描述（由申请人提供）：Sigma-2受体是在大多数哺乳动物组织中发现的独特蛋白质。σ-2受体在神经元和非神经元来源的正常细胞和肿瘤细胞的生长调节中起重要作用。Sigma-2受体尚未被纯化或克隆。这项拨款建议是针对σ-2受体的纯化和克隆。这一目标将通过两种交替和互补的办法来实现。具体目标1的标题为：使用“点击化学”方法纯化、鉴定和克隆Sigma-2受体。具体目标2的标题是：使用计算机模拟方法鉴定和克隆Sigma-2受体。σ-2受体的表征是导致对σ-2受体在调节神经元和非神经元细胞功能中的作用的基本理解以及用于治疗药物滥用和/或异常细胞生长的潜在治疗策略的初始步骤。.公共卫生相关性：sigma-2受体是一种哺乳动物中枢神经系统和外周器官中的蛋白质，可能与药物成瘾有关。σ-2受体在正常细胞和不同来源的肿瘤细胞中的生长调节中起重要作用。尽管存在许多分子生物学工具，但迄今为止，sigma-2受体成功地避开了所有克隆尝试。该项目旨在纯化和鉴定sigma-2受体。

DESCRIPTION (provided by applicant): The Sigma-2 receptor is a unique protein found in most mammalian tissues. Sigma-2 receptors play an important role in growth regulation in normal cells and tumor cells of neuronal and non neuronal origin. The Sigma-2 receptor has not been purified or cloned. This grant proposal is directed at purification and cloning of the Sigma-2 receptor. This goal will be accomplished through two alternate and complementary approaches. Specific Aim 1 is entitled: Purification, Identification and Cloning of the Sigma-2 receptor using a "Click Chemistry" approach. Specific Aim 2 is entitled: Identification and cloning of the Sigma-2 receptor using an In Silico approach. Characterization of the Sigma-2 receptor is the initial step leading to a basic understanding of the role of the Sigma-2 receptor in regulation of neuronal and non-neuronal cell function and for potential therapeutic strategies to treat drugs of abuse and/or aberrant cell growth. . PUBLIC HEALTH RELEVANCE: The sigma-2 receptor is a mammalian protein in the central nervous system and in peripheral organs that may be involved in drug addiction. Sigma-2 receptors play an important role in growth regulation in normal cells and in tumor cells of different origins. Despite the presence of numerous molecular biology tools, the sigma-2 receptor has managed to evade all cloning attempts to date. This project is directed at purification and identification of the sigma-2 receptor.