Flow Cytometry Laboratory (Scientific Core)

虽然流式细胞术实验室是位于巴尔的摩NIA校内研究计划的实验室科学任务的核心，但几个研究人员，实验室小组或项目占2008财年资源的很大一部分。免疫学实验室（LI; Taub, Weng, Sen）的使用在40-60%的分选和50-70%的非分选（分析）能力之间变化，重点是人类和动物（小鼠）的免疫状态、免疫调节、免疫抑制模型及其与发育和衰老的关系。其他支持的项目包括心血管科学实验室(LCS: Boheler， ES细胞和心肌细胞分化；遗传学实验室（LG: Ko， ES发育模式），神经科学实验室（LNS: Mattson，神经干细胞和神经发生中的报告基因表达），临床研究实验室（Bernier），分子老年学实验室（LMG: Gearhart，抗体多样性和体细胞超突变），细胞和分子生物学实验室（LCMB; Evans，活性氧的功能分析）。虽然实验室不要求我们为其生成数据的所有出版物都是共同作者，但除了下面的参考书目之外，以下列表还列出了IRP的出版物，其中包括我们核心在本报告期间的贡献。

While the Flow Cytometry Laboratory functions as a core for the scientific mission of the laboratories located within the NIA Intramural Research Program in Baltimore, several investigators, laboratory groups, or projects accounts for a significant portion of FY2008 resources. Usage by the Laboratory of Immunology (LI; Taub, Weng, Sen) varies between 40-60% of sorting and 50-70% of non-sorting (analytical) capacities, focusing on human and animal (mouse) models of immune status, immunoregulation, immunosuppression, and their relation to development and aging. Other supported projects involve those from the Laboratory of Cardiovascular Sciences (LCS: Boheler, ES cells and cardiomyocte differentiation; Xiao, reporter gene expression), Laboratory of Genetics (LG: Ko, patterns of ES development), Laboratory of Neuroscience (LNS: Mattson, reporter gene expression in neural stem cells and neurogenesis), Laboratory of Clinical Investigation (Bernier), Laboratory of Molecular Gerontology (LMG: Gearhart, antibody diversity and somatic hypermuations), and Laboratory of Cellular and Molecular Biology (LCMB; Evans; functional analysis of reactive oxygen species). While the lab does not require coauthorship on all publications for which we generate data, the following list, in addition to the bibliography below, documents publications from the IRP that include contributions from our core for this reporting period. 1) Dawson HD, Collins G, Pyle R, Key M, Taub DD. (2008). The retinoic acid receptor-alpha mediates human T-cell activation and Th2 cytokine and chemokine production. BMC Immunol 9:16. 2) Dixit, VD, Yang Y, Sun Y, Weeraratna AT, Youm YH, Smith RG, Taub DD. (2007). Ghrelin promotes thymopoiesis during aging. J Clin Invest 117:2778-90. 3) Heltemes-Harris LM, Gearhart PJ, Ghosh P, Longo DL. (2008). Activation-induced deaminase mediated class switch recombination is blocked by anti-IgM signaling in a phosphatidyl-insotitol 3-kinase dependent fashion. Mol. Immunol 45:1799-06. 4) Hill JW, Hu JJ, Evans MK. (2008). OGG1 is degraded by calpain following oxidative stress and cisplatin exposure DNA Repair 7:648-654. 5) Hossain MZ, Yu Q, Xu M, Sen JM. (2008). ICAT expression disrupts beta-catenin-TCF interactions and impairs survival of thymocytes and activated mature T cells. Int Immunol 20:925-35. 6) Kleawsongkram J, Yang Y, Golech S, Katz J, Kaestner KH, Weng NP. (2007). Krupple-like factor 4 regulates B cell number and activation-induced b cell proliferation. J Immuunol 179:4679-4684. 7) Lathia JD, Patton B, Eckley DM, Magnus T, Mughal MR, Sasaki T, Caldwell MA, Rao MS, Mattson MP, French-Constant C. (2007). Patterns of extracellular matrix/intergriin expression in the embryonic ventricular zone of the CNS J Comparative Neurology 505:630-643. 8) Martomo SA, Saribasak H, Yokoi M, Hanaoka F, Gearhart PJ. (2008). Re-evaluation of the role of DNA polymerae theta in somatic hypermutation of immunoglobulin genes DNA Repair 7:1603-8. 9) Yang Y, An J, Weng NP. (2008). Telomerase is involved in IL-7-mediated differential survival of naive and memory CD4+ T cells. J Immunol 180:3775-81. 10) Yu Q, Quinn WJ 3rd, Salay T, Crowley JE, Cancro MP, Sen JM. (2008). Role of beta-catenin in B cell development and function. J Immunol 181:3777-83. 11) Xu M, Yu Q, Subrahmanyam R, Difilippantonio MJ, Ried T, Sen JM. (2008). Beta-catenin expression results in p53-independent DNA damage and oncogene-induced senescence in prelymphomagenic thymocytes in vivo. Mol Cell Biol 28:1713-23.