TUSM/T-NEMC CENTER FOR NEUROSCIENCE RESEARCH - Imaging Core

理由和使命。先进显微技术的发展是 生物研究和医学成像的进展。在过去的几十年里， 共焦/多光子显微镜的发展沿着荧光团的发展（如 GFP衍生物，变色龙和许多其他的）已经导致了生物显微镜的复兴（Denk et 例如，Science 248，1990; Piston，Trends Cell Biol 9，1999; Diaspro，2002，Wiley-Liss;货车Roessel和Brand， Nat Cell Biol 4，2002; Giepmans等人，Science 312，2006）。然而，最新的成像方法通常需要 这是一个巨大的挑战，因为它需要超出大多数科学家个人的财政和技术能力的专业知识和工具。因此，在本发明中， 共享的成像设备已成为大学研究核心的重要组成部分。 CNR成像和细胞分析核心是为了应对当代医学的快速发展而开发的。 生物显微镜和满足塔夫茨神经科学研究人员的成像优先权。的 核心现在为塔夫茨神经科学家提供广泛的显微成像服务，包括宽视野 荧光显微镜和数字成像，共聚焦显微镜，双光子显微镜，活细胞成像， 电子显微镜和激光捕获显微切割。除了标准 微观调查，调查人员使用核心设施进行动态 成像研究如荧光共振能量转移（FRET）或 光漂白后的荧光恢复（FRAP）实验。核心 设备和服务将在后面的章节中描述。

Rationale and Mission. The development of advanced microscopy techniques is a driving force behind advances in biological research and medical imaging. In the past several decades, the refinement and commercialization of confocal/multiphoton microscopes along with advances in fluorophore development (such as GFP derivatives, Chameleons and many others) have led to a renaissance in biological microscopy (Denk et al., Science 248, 1990; Piston, Trends Cell Biol 9, 1999; Diaspro, 2002, Wiley-Liss; Van Roessel and Brand, Nat Cell Biol 4, 2002; Giepmans et al., Science 312, 2006). The newest imaging methods, however, often require expertise and instruments beyond the financial and technical capacity of most individual scientists. Thus, shared imaging facilities have become crucial components of university research cores. The CNR Imaging & Cell Analysis Core was developed in response to the rapid advances occurring in contemporary biological microscopy and to meet the imaging priorities of Tufts neuroscience investigators. The core now provides Tufts neuroscientists with a broad range of microscopic imaging services including widefield fluorescence microscopy & digital imaging, confocal microscopy, 2-photon microscopy, live-cell imaging, electron microscopy, and laser capture microdissection. In addition to standard microscopic investigations, investigators use core facilities to perform dynamic imaging studies such as fluorescence resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP) experiments. Core equipment and services are described in a later section.