TRANSGENIC MOUSE/ ES CELL SHARES RESOURCES

转基因小鼠/ES细胞共享资源促进了转基因小鼠/ES细胞的产生、维护和保存 转基因小鼠，从而为研究糖尿病、肥胖症和 控制碳水化合物、脂肪和蛋白质动态平衡的机制。这个设施已经投入使用， 由DRTC支持超过12年，目前提供九种不同的服务 转基因小鼠的生产和维护。在其历史上，转基因小鼠/胚胎干细胞 共享资源已经为131名不同的研究人员服务，产生了超过1755只转基因创始小鼠 来自482个不同的DNA结构和来自203个不同ES细胞克隆的2566只嵌合小鼠。共享的 进一步协助培育了至少101只不同的转基因小鼠 胚胎干细胞中的同源重组。许多转基因小鼠已经被 被范德比尔特和其他机构的研究人员用来深入了解胰岛的功能， 研究对象包括肝脏、骨骼肌和脂肪，并研究糖尿病的并发症，如糖尿病肾病。其他 这个核心提供的服务包括辅助生殖，以维持不再是 育种、胚胎和精子冷冻，以及条件等位基因的Cre和Flpe操作。通过 与DRTC附属调查人员密切合作，为突变小鼠提供建议和指导 生产、筛选策略、维护和分析所产生的动物，该设施提供 最先进的、经济高效的、质量受控的、高效的遗传生成方法 为DRTC附属研究人员改造的小鼠。这一核心是范德比尔特共享设施系统的一部分， 它提供了一个高效的计费系统以及对核心的监督和治理。这一核心将继续下去 在下一笔资金中提供必要的服务，支持DRTC附属调查人员的研究 周而复始。

The Transgenic Mouse/ES Cell Shared Resource facilitates the generation, maintenance, and storage of genetically modified mice, thereby providing useful animal models for the study of diabetes, obesity, and the mechanisms controlling carbohydrate, fat, and protein homeostasis. This facility has been in operation and supported by the DRTC for over 12 years and currently provides nine different services related to the production and maintenance of genetically modified mice. During its history, the Transgenic Mouse/ES Cell Shared Resource has served 131 different investigators by generating over 1755 transgenic founder mice from 482 distinct DNA constructs and 2566 chimeric mice from 203 different ES cell clones. The Shared Resource has further assisted in the generation of at least 101 different genetically modified mice using homologous recombination in embryonic stem (ES) cells. Many of the genetically modified mice have been used by investigators at Vanderbilt and other institutions to gain insight into the function of pancreatic islets, the liver, skeletal muscle, and fat and to study complications of diabetes such as diabetic nephropathy. Other services provide by this core include assisted reproduction to maintain founder lines that are no longer breeding, embryo and sperm cryopreservation, and Cre and Flpe manipulation of conditional alleles. By working closely with DRTC-affiliated investigators to provide advice and instruction on mutant mouse production, screening strategies, maintenance, and analysis of the animals generated, the facility provides state-of-the-art, cost-effective, quality-controlled, and efficient approaches for the generation of genetically modified mice for DRTC-affiliated investigators. This core is part of the Vanderbilt shared facilities system, which provides an efficient billing system and oversight and governance for the core. This core will continue to provide essential services that support the research of DRTC-affiliated investigators in the next funding cycle.