FUNCTIONAL ANALYSIS OF COMPLEMENT RECEPTOR 2 AS A LUPUS SUSCEPTIBILITY GENE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 补体受体2是抗体产生细胞上的一种蛋白质，被认为在狼疮中起着重要作用。我们已经证明，在狼疮的动物模型中，这种蛋白的基因是异常的，这为它导致狼疮提供了强有力的证据。我们还发现，这种蛋白基因的某些变异在狼疮患者中更常见。在这项研究中，我们想要确定补体受体2基因的变异如何影响其功能，以便我们能够更好地了解它可能如何参与狼疮。为了做到这一点，我们将从表达不同版本基因的不同人身上提取血液，以多种不同的方式测试受体的功能。此外，我们将从一些人身上获取一小片扁桃体，以测试某种形式的受体的功能，这种受体在扁桃体中表达，但不在血液中表达。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Complement receptor 2 is a protein on antibody-producing cells believed to be important in lupus. We have shown that the gene for this protein is abnormal in an animal model of lupus, providing strong proof that it causes lupus. We have also shown that certain variations in the gene for this protein are found more often in individuals who have lupus. In this study, we want to determine how the variations in the complement receptor 2 gene affect its function so that we can understand better how it might be involved in lupus. To do this, we will draw blood from different people who express different versions of the gene to test the function of the receptor in a number of different ways. In addition, we will obtain a small piece of the tonsils from some people to test the function of a form of the receptor that is expressed in the tonsils but not in the blood.