Genetic and molecular approaches to cardiovascular disease

描述(由申请人提供)： 这一建议源于劳伦斯伯克利国家实验室由NHLBI资助的长期博士后培训计划，该计划随着时间的推移不断发展，以保持对心血管研究最前沿主题的关注，并培养了许多具有广泛背景的科学家，他们已被学术界和工业界积极招聘。这个多学科项目的领导和管理将转移到儿童医院奥克兰研究所，并将保留劳伦斯伯克利国家实验室的一些原始教师。培训的一个主要重点是使用基因组技术来研究动脉粥样硬化性心血管疾病的机制。这八位项目导师每个人都经营着成功且资金充足的实验室，并带来了脂蛋白代谢、动脉粥样硬化和心血管生物学的细胞、分子、生物物理、计算、遗传和基因组方面的不同和互补的专业知识。我们提供最先进领域的培训，如操纵小鼠基因组，下一代测序，序列数据的计算分析，遗传关联分析，人类序列变异的计算分析，以预测其在疾病中的潜在作用，基因表达谱，表观遗传学，以及脂蛋白结构的生物物理研究。此外，基因组学方法非常重视研究基因调控，重点放在心血管系统，以及重新测序和基因分型，以确定导致心血管疾病的人类序列变异。我们位于一家大型研究机构内，距离加州大学伯克利分校很近，创造了一个丰富的培训环境，有可能进行许多合作和培训机会，超出了这笔赠款的范围。受训者接受的跨学科培训将为他们提供开发独立研究计划的技能，这些计划利用现代生物医学研究所需的实验和计算方法。相关性(见说明)：这项建议支持对下一代科学家的培训，他们将了解心血管疾病的遗传和生化基础。

DESCRIPTION (provided by applicant): This proposal stems from a long-standing NHLBI-funded post-doctoral training program based at the Lawrence Berkeley National Laboratory that has evolved over time to maintain focus on the most cutting edge topics in cardiovascular research, and has trained many scientists with a wide range of backgrounds who have been actively recruited by academia as well as industry. The leadership and management of this multidisciplinary program will move to Children's Hospital Oakland Research Institute, and it will retain some of the original faculty from the Lawrence Berkeley National Laboratory. A major emphasis of the training is the use of genomic technologies to investigate mechanisms involved in atherosclerotic cardiovascular disease. The eight program mentors each run successful and well-funded laboratories, and bring varied and complementary expertise in cellular, molecular, biophysical, computational, genetic, and genomic aspects of lipoprotein metabolism, atherogenesis and cardiovascular biology. We offer training in state-of-the art areas such as manipulation the genomes of mice, next-generation sequencing, computational analysis of sequence data, analysis of genetic association, computational analysis of human sequence variation to predict its potential role in disease, gene expression profiling, and epigenetics, as well as biophysical studies of lipoprotein structure. In addition, there is strong emphasis on genomic approaches to study gene regulation with a focus on the cardiovascular system and on resequencing and genotyping to identify human sequence variation responsible for cardiovascular disorders. Our location within a major research institution and in close proximity to the University of California, Berkeley creates a rich training environment with potential for numerous collaborations and training opportunities beyond the scope of this grant. The cross- disciplinary training that the trainees receive will provide them with the skills to develop independent research programs that exploit the experimental and computational approaches required for modern biomedical research. RELEVANCE (See instructions): This proposal supports the training of the next generation of scientist who will understand the genetic and biochemical basis of cardiovascular disease.