STRUCTURAL CHARACTERIZATION OF THE TYPE 3 SECRETION SYSTEM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们计划使用自然质谱学来确定一个非常大的(&gt；2MDa)跨膜蛋白复合体--3型分泌系统的亚单位化学计量比。3型分泌系统参与了沙门氏菌等病原体对毒力因子的注射。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are planning to use native mass spectrometry to determine the subunit stoichiometry of a very large (>2MDa) membrane spanning protein complex - the Type 3 Secretion System. The Type 3 Secretion System is involved in injection of virulence factors by pathogen such as salmonella.