Dynamics of Hair Bundle Proteins

毛细胞机械转导是我们听觉和平衡的基础过程。的关注点 Shin实验室研究的是调节极其敏感的机械转导过程的蛋白质。 应激因素，如反应性自由基和机械过度刺激，会损害毛细胞蛋白质， 这就提出了这样一个问题：这些蛋白质是如何修复或替换的？我们的假设是， 维持毛细胞蛋白质的完整性是蛋白质持续周转的过程。因此，我感兴趣的是 毛细胞蛋白质的动态周转，特别是在应对有害影响时，如氧化应激和 噪音损坏。我现在的RoO提案的目标是使用代谢的质谱分析 标记蛋白质和毛束蛋白质的体内成像研究毛细胞的周转和动态 蛋白质。 莱文摘要：听力器官的健康有赖于其分子的不断更替 组件。拟议的研究使用质谱学和成像技术来解决 质疑噪音损害等有害环境刺激是否会影响蛋白质的周转 对听力很重要。这项研究的结果将有助于开发预防和恢复听力的方法 人类的损失。

Hair cell mechanotransduction is the process that underlies our senses of hearing and balance. The focus of the Shin lab is the study of proteins that mediate the exquisitely sensitive mechanotransduction process. Stress factors such as reactive radicals and mechanical overstimulation can damage hair cell proteins, raising the question how these proteins are repaired or replaced. Our hypothesis is that the main strategy for maintaining integrity of hair ceil proteins is a constant turnover of proteins. I am therefore interested in the dynamic turnover of hair ceil proteins, especially in response to harmful impacts such as oxidative stress and noise damage. The goal of my present ROO proposal is to use mass spectrometry analysis of metabolically labeled proteins and in vivo imaging of hair bundle proteins to study turnover and dynamics of hair cell proteins. Lay summary: The health of the hearing organ depends on the constant replacement of its molecular components. The proposed study uses mass spectrometry and imaging technologies to address the question whether harmful environmental stimuli such as noise damage can influence the turnover of proteins important for hearing. The results of this study wiii help to develop methods to prevent and restore hearing losses in humans.